ArticleCell systems2017
Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis.
Article in Cell systems, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 422 papers.
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422 citing papers in PubMed.
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- The alarmone pGpp inhibits translation through GTP depletion.Nucleic acids research · 2026Article
- Quinone-transporting filaments expand bioenergetic capacity in Gram-positive Bacillota.Nature microbiology · 2026Article
- Molecular insights into the promiscuous ApThe Journal of biological chemistry · 2026Article
- Genome shuffling enables quantitative trait locus mapping in Bacillus subtilis.Nature communications · 2026Article
- YpeB stability affects germination possibly through delaying SleB activity inJournal of bacteriology · 2026Article
- Simultaneous measurements of translation rate and transcriptome uncovers linked regulation within an active bacterial cell population.Science advances · 2026Article
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- Processing of reversed replication forks is required for the resolution of replication-transcription conflicts.Nucleic acids research · 2026Article
- Exploring the metabolic burden of surfactin biosynthesis and the metabolic costs of srfA operon expression in Bacillus subtilis.Microbial cell factories · 2026Article
- Mfd interacts with RNA polymerase to modulate flagellum-dependent motility, chemotaxis, and population heterogeneity in the stationary phase ofJournal of bacteriology · 2026Article
362 more citing papers are in PubMed but not listed here.
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Authors and funding
15 authors.
Funding
Abstract
A systems-level understanding of Gram-positive bacteria is important from both an environmental and health perspective and is most easily obtained when high-quality, validated genomic resources are available. To this end, we constructed two ordered, barcoded, erythromycin-resistance- and kanamycin-resistance-marked single-gene deletion libraries of the Gram-positive model organism, Bacillus subtilis. The libraries comprise 3,968 and 3,970 genes, respectively, and overlap in all but four genes. Using these libraries, we update the set of essential genes known for this organism, provide a comprehensive compendium of B. subtilis auxotrophic genes, and identify genes required for utilizing specific carbon and nitrogen sources, as well as those required for growth at low temperature. We report the identification of enzymes catalyzing several missing steps in amino acid biosynthesis. Finally, we describe a suite of high-throughput phenotyping methodologies and apply them to provide a genome-wide analysis of competence and sporulation. Altogether, we provide versatile resources for studying gene function and pathway and network architecture in Gram-positive bacteria.
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