Evidence map›Paper›PMID 28188593›Full record

ArticleJournal of assisted reproduction and genetics2017

Re-analysis of aneuploidy blastocysts with an inner cell mass and different regional trophectoderm cells.

Jin Huang, Liying Yan, Sijia Lu, Nan Zhao, Jie Qiao

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In one paragraph

Article in Journal of assisted reproduction and genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 2 syntheses or guidelines pooled it.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Jin HuangReproductive Medical Centre, Department of Obstetrics and Gynecology, Peking University Third Hospital, 49 Huayuan North Rd, Haidian, Beijing, China.
Liying YanReproductive Medical Centre, Department of Obstetrics and Gynecology, Peking University Third Hospital, 49 Huayuan North Rd, Haidian, Beijing, China.
Sijia LuYikon Genomics, Beijing, China.
Nan ZhaoReproductive Medical Centre, Department of Obstetrics and Gynecology, Peking University Third Hospital, 49 Huayuan North Rd, Haidian, Beijing, China.
Jie QiaoReproductive Medical Centre, Department of Obstetrics and Gynecology, Peking University Third Hospital, 49 Huayuan North Rd, Haidian, Beijing, China. jie.qiao@263.net.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeThe purpose of this study is to explore which part of the trophectoderm best represents the inner cell mass after aCGH analysis.

methodsFifty-one preimplantation genetic diagnosis/preimplantation genetic screening of abnormal blastocysts diagnosed by array comparative genomic hybridization were included in this study. Blastocysts were thawed, incubated for 3 to 4 h, and then biopsied. Four regions were biopsied per blastocyst, including the inner cell mass (ICM), trophectoderm (TE) cells opposite the ICM, TE cells at the upper right of the ICM, and TE cells at the lower right of the ICM. The biopsied pieces were processed through multiple annealing and looping-based amplification cycle sequenced for 24-chromosome aneuploidy screening. The aneuploidy results were compared among the ICM and the different regional trophectoderm cells from the same blastocyst.

resultsFifty of 51 (98.04%) ICM samples were concordant with at least one of the TE biopsies derived from the same embryos. There were 43 blastocysts in which ICM and the other three TE pieces were consistent. Discordance among the four pieces occurred in eight blastocysts. Only one blastocyst was discordant between the ICM and the other three TE pieces, while seven blastocysts were discordant between one of TE and the other three biopsied pieces. There was no special region that the mosaic TE was located.

conclusionsOur findings indicate that TE aneuploidy is an excellent predictor of ICM aneuploidy. The blastocyst mosaic cells are inclined to be located in TE. Moreover, the mosaic TE was not limited to the special region.

Indexed as

Comparative Genomic HybridizationPreimplantation DiagnosisAneuploidyBiopsyBlastocystBlastocyst Inner Cell MassFemaleFertilization in VitroHumansMosaicismPregnancy24-chromosome aneuploidy screeningBlastocystMosaicMultiple annealing and looping-based amplification cycle sequencingPreimplantation genetic screening

Identifiers

PMID28188593
PMCPMC5401696

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.