Evidence map›Paper›PMID 28187768›Full record

ArticleMicrobial cell factories2017

Current lipid extraction methods are significantly enhanced adding a water treatment step in Chlorella protothecoides.

Xiaojie Ren, Xinhe Zhao, François Turcotte, Jean-Sébastien Deschênes, Réjean Tremblay, Mario Jolicoeur

Open access · goldAbstract read
In one paragraph

Article in Microbial cell factories, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 80 citations in OpenAlex.

  1. Extracellular vesicles ofGut microbes · 2025
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  4. Enhancing Biomass and Lipid Production inLife (Basel, Switzerland) · 2025
    Article
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  12. Potential of cyanobacteria in the conversion of wastewater to biofuels.World journal of microbiology & biotechnology · 2021
    Review
  13. Article
  14. Protective Effect of a Mixture ofEvidence-based complementary and alternative medicine : eCAM · 2020
    Article
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Xiaojie RenResearch Laboratory in Applied Metabolic Engineering, Department of Chemical Engineering, École Polytechnique de Montreal, P.O. Box 6079, Centre-ville Station, Montreal, QC, H3C 3A7, Canada.
Xinhe ZhaoResearch Laboratory in Applied Metabolic Engineering, Department of Chemical Engineering, École Polytechnique de Montreal, P.O. Box 6079, Centre-ville Station, Montreal, QC, H3C 3A7, Canada.
François TurcotteUniversité du Québec à Rimouski, 310 allée des Ursulines, Rimouski, QC, G5L 3A1, Canada.
Jean-Sébastien DeschênesUniversité du Québec à Rimouski, 310 allée des Ursulines, Rimouski, QC, G5L 3A1, Canada.
Réjean TremblayUniversité du Québec à Rimouski, 310 allée des Ursulines, Rimouski, QC, G5L 3A1, Canada.
Mario JolicoeurResearch Laboratory in Applied Metabolic Engineering, Department of Chemical Engineering, École Polytechnique de Montreal, P.O. Box 6079, Centre-ville Station, Montreal, QC, H3C 3A7, Canada. mario.jolicoeur@polymtl.ca.
Polytechnique Montréal · CAUniversité du Québec à Rimouski · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMicroalgae have the potential to rapidly accumulate lipids of high interest for the food, cosmetics, pharmaceutical and energy (e.g. biodiesel) industries. However, current lipid extraction methods show efficiency limitation and until now, extraction protocols have not been fully optimized for specific lipid compounds. The present study thus presents a novel lipid extraction method, consisting in the addition of a water treatment of biomass between the two-stage solvent extraction steps of current extraction methods. The resulting modified method not only enhances lipid extraction efficiency, but also yields a higher triacylglycerols (TAG) ratio, which is highly desirable for biodiesel production.

resultsModification of four existing methods using acetone, chloroform/methanol (Chl/Met), chloroform/methanol/H

conclusionWater treatment of biomass after the first solvent extraction step helps the subsequent release of intracellular lipids in the second extraction step, thus improving the global lipids extraction yield. In addition, the water treatment positively modifies the intracellular lipid class ratios of the final extract, in which TAG ratio is significantly increased without changes in the fatty acids composition. The novel method thus provides an efficient way to improve lipid extraction yield of existing methods, as well as selectively favoring TAG, a lipid of the upmost interest for biodiesel production.

Indexed as

BiofuelsBiomassCell FractionationChlorellaFatty AcidsLipidsLiquid-Liquid ExtractionMethanolSolventsTriglyceridesWaterBiofuelsFatty AcidsLipidsMethanolSolventsTriglyceridesWaterChlorella protothecoidesHigh extraction yieldHigh TAG ratioLipid extractionTwo-stage solvent extractionsWater treatment

Identifiers

PMID28187768
PMCPMC5303247
OpenAlexW2586375435

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.