ArticlePloS one2017
Laboratory strains of Aedes aegypti are competent to Brazilian Zika virus.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 35 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Zika virus: An updated review of competent or naturally infected mosquitoes.PLoS neglected tropical diseases · 2017Pooled it
- The toolbox for mosquito vector research.Parasites & vectors · 2025Review
- Sampling efficiency and screening ofThe Pan African medical journal · 2024Article
- Transgene-induced cell death following dengue-2 virus infection in Aedes aegypti.Scientific reports · 2023Article
- Evaluation of Methods to Collect Diurnal Culicidae (Diptera) at Canopy and Ground Strata, in the Atlantic Forest Biome.Insects · 2022Article
- Genetic Stability and Fitness ofFrontiers in bioengineering and biotechnology · 2022Article
- Natural Infection and Vertical Transmission of Zika Virus in Sylvatic MosquitoesTropical medicine and infectious disease · 2021Article
- Effect of long-term exposure to copper on survival and development of two successive generations of Culex pipiens (Diptera, Culicidae).Ecotoxicology (London, England) · 2021Article
- Genetic sexing strains for the population suppression of the mosquito vectorPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Article
- House-Level Risk Factors forJournal of tropical medicine · 2021Article
- Olfaction-Related Gene Expression in the Antennae of Female Mosquitoes From CommonFrontiers in physiology · 2021Article
- Multiple dengue virus serotypes resistant transgenicRNA biology · 2020Article
- Age-structured vectorial capacity reveals timing, not magnitude of within-mosquito dynamics is critical for arbovirus fitness assessment.Parasites & vectors · 2020Article
- Maternal Zika Virus (ZIKV) Infection following Vaginal Inoculation with ZIKV-Infected Semen in Timed-Pregnant Olive Baboons.Journal of virology · 2020Article
- One-step RT-qPCR assay for ZIKV RNA detection in Aedes aegypti samples: a protocol to study infection and gene expression during ZIKV infection.Parasites & vectors · 2020Article
- Impact of temperature on the extrinsic incubation period of Zika virus in Aedes aegypti.PLoS neglected tropical diseases · 2020Article
- Vector Competence: What Has Zika Virus Taught Us?Viruses · 2019Review
- Transgenic Aedes aegypti Mosquitoes Transfer Genes into a Natural Population.Scientific reports · 2019Article
- Vertical transmission of Zika virus in Culex quinquefasciatus Say and Aedes aegypti (L.) mosquitoes.Scientific reports · 2019Article
- Development and Validation of Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) for Rapid Detection of ZIKV in Mosquito Samples from Brazil.Scientific reports · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
9 authors.
Funding
Abstract
The Zika virus outbreaks are unprecedented human threat in relation to congenital malformations and neurological/autoimmune complications. Since this virus has high potential to spread in regions presenting the vectors, improvement in mosquito control is a top priority. Thus, Aedes aegypti laboratory strains will be fundamental to support studies in different research fields implicated on Zika-mosquito interactions which are the basis for the development of innovative control methods. In this sense, our aim was to determine the main infection aspects of a Brazilian Zika strain in reference Aedes aegypti laboratory mosquitoes. We orally exposed Rockefeller, Higgs and Rexville mosquitoes to the Brazilian ZIKV (ZIKVBR) and qRT-PCR was applied to determine the infection, dissemination and detection rates of ZIKV in the collected saliva as well as viral levels in mosquito tissues. The three strains sustain the virus development but Higgs showed significantly lower viral loads in bodies at 14 days post-infection (dpi) and the lowest prevalences in bodies and heads. The Rockefeller strain was the most susceptible at 7 dpi but similar dissemination rates were observed at 14 dpi. Although variations exist, the ZIKVBR RNA shows detectable levels in saliva of the three strains at 14 dpi but is only detected in Rockefeller at 7 dpi. Moreover, saliva samples from the three strains were confirmed to be infectious when intrathoracically injected into mosquitoes. The ZIKVBR kinetics was monitored in Rockefeller mosquitoes and virus could be identified in the heads at 4 dpi but was more consistently detected late in infection. Our study presents the first evaluation on how Brazilian Zika virus behaves in reference Aedes aegypti strains and shed light on how the infection evolves over time. Vector competence and hallmarks of the ZIKVBR development were revealed in laboratory mosquitoes, providing additional information to accelerate studies focused on ZIKV-mosquito interactions.
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