ArticleScientific reports2017
Complexity of the Ruminococcus flavefaciens FD-1 cellulosome reflects an expansion of family-related protein-protein interactions.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 48 citations in OpenAlex.
- Article
- Article
- Niche specialization and cross-feeding interactions shaping gut microbial fiber degradation in a model omnivore.bioRxiv : the preprint server for biology · 2026Article
- Advancing cellulose degradation through synthetic biology: engineered pathways and microbial systems for sustainable biomass conversion.Journal of animal science and biotechnology · 2026Review
- Artificial polysaccharide-binding protein mediates co-assembly of nanodrug and probiotics against drug-resistant infectious enteritis.Asian journal of pharmaceutical sciences · 2025Article
- Lignocellulose degradation in bacteria and fungi: cellulosomes and industrial relevance.Frontiers in microbiology · 2025Review
- Investigation of Intestinal Microbes of Five Zokor Species Based on 16S rRNA Sequences.Microorganisms · 2024Article
- Important roles ofFood science and biotechnology · 2024Review
- A cellulosomal double-dockerin module from Clostridium thermocellum shows distinct structural and cohesin-binding features.Protein science : a publication of the Protein Society · 2024Article
- Cryptic diversity of cellulose-degrading gut bacteria in industrialized humans.Science (New York, N.Y.) · 2024Article
- Purified fibers in chemically defined synthetic diets destabilize the gut microbiome of an omnivorous insect model.Frontiers in microbiomes · 2024Article
- Degradation of Cellulose and Hemicellulose by Ruminal Microorganisms.Microorganisms · 2022Review
- Understanding the Role ofAnimals : an open access journal from MDPI · 2021Article
- Functional differentiation related to decomposing complex carbohydrates of intestinal microbes between two wild zokor species based on 16SrRNA sequences.BMC veterinary research · 2021Article
- Constructing an EfficientMolecules (Basel, Switzerland) · 2021Article
- Proteome specialization of anaerobic fungi during ruminal degradation of recalcitrant plant fiber.The ISME journal · 2021Article
- Cellulosomes: Highly Efficient Cellulolytic Complexes.Sub-cellular biochemistry · 2021Review
- Dietary osteopontin-enriched algal protein as nutritional support in weaned pigs infected with F18-fimbriated enterotoxigenic Escherichia coli.Journal of animal science · 2020Article
- Multi-omic Directed Discovery of Cellulosomes, Polysaccharide Utilization Loci, and Lignocellulases from an Enriched Rumen Anaerobic Consortium.Applied and environmental microbiology · 2020Article
- Mechanisms of Nanonewton Mechanostability in a Protein Complex Revealed by Molecular Dynamics Simulations and Single-Molecule Force Spectroscopy.Journal of the American Chemical Society · 2019Article
Corrections and comments
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Authors and funding
18 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein-protein interactions play a vital role in cellular processes as exemplified by assembly of the intricate multi-enzyme cellulosome complex. Cellulosomes are assembled by selective high-affinity binding of enzyme-borne dockerin modules to repeated cohesin modules of structural proteins termed scaffoldins. Recent sequencing of the fiber-degrading Ruminococcus flavefaciens FD-1 genome revealed a particularly elaborate cellulosome system. In total, 223 dockerin-bearing ORFs potentially involved in cellulosome assembly and a variety of multi-modular scaffoldins were identified, and the dockerins were classified into six major groups. Here, extensive screening employing three complementary medium- to high-throughput platforms was used to characterize the different cohesin-dockerin specificities. The platforms included (i) cellulose-coated microarray assay, (ii) enzyme-linked immunosorbent assay (ELISA) and (iii) in-vivo co-expression and screening in Escherichia coli. The data revealed a collection of unique cohesin-dockerin interactions and support the functional relevance of dockerin classification into groups. In contrast to observations reported previously, a dual-binding mode is involved in cellulosome cell-surface attachment, whereas single-binding interactions operate for cellulosome integration of enzymes. This sui generis cellulosome model enhances our understanding of the mechanisms governing the remarkable ability of R. flavefaciens to degrade carbohydrates in the bovine rumen and provides a basis for constructing efficient nano-machines applied to biological processes.
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