ArticleScientific reports2017
All-in-one 3D printed microscopy chamber for multidimensional imaging, the UniverSlide.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Custom 3D-Printed Molds for Zebrafish Imaging and Cardiac Development.Journal of visualized experiments : JoVE · 2025Article
- A method for creating custom 3D-printed molds to facilitate zebrafish imaging studies, including of cardiac development.bioRxiv : the preprint server for biology · 2025Article
- Agarose stamped method: a simple and customizable immobilization technique for zebrafish larvae.Frontiers in behavioral neuroscience · 2025Article
- Cells-in-Touch: 3D Printing in Reconstruction and Modelling of Microscopic Biological Geometries for Education and Future Research Applications.Bioengineering (Basel, Switzerland) · 2023Article
- TheRoyal Society open science · 2022Article
- Mod3D: A low-cost, flexible modular system of live-cell microscopy chambers and holders.PloS one · 2022Article
- Picroscope: low-cost system for simultaneous longitudinal biological imaging.Communications biology · 2021Article
- Tools to Image Germplasm Dynamics During Early Zebrafish Development.Frontiers in cell and developmental biology · 2021Article
- 3D-printed Bioreactors forInternational journal of bioprinting · 2020Review
- Manipulating cultured mammalian cells for mitosis research.Methods in cell biology · 2020Article
- Standardized mounting method of (zebrafish) embryos using a 3D-printed stamp for high-content, semi-automated confocal imaging.BMC biotechnology · 2019Article
- A model of guided cell self-organization for rapid and spontaneous formation of functional vessels.Science advances · 2019Article
- A Smart Imaging Workflow for Organ-Specific Screening in a Cystic Kidney Zebrafish Disease Model.International journal of molecular sciences · 2019Article
- 3D printing in cell culture systems and medical applications.Applied physics reviews · 2018Review
- 3D-printed miniaturized fluidic tools in chemistry and biology.Trends in analytical chemistry : TRAC · 2018Article
- High-throughput live-imaging of embryos in microwell arrays using a modular specimen mounting system.Biology open · 2018Article
- MineLoC: A Rapid Production of Lab-on-a-Chip Biosensors Using 3D Printer and the Sandbox Game, Minecraft.Sensors (Basel, Switzerland) · 2018Article
- Zebrafish: an emerging real-time model system to study Alzheimer's disease and neurospecific drug discovery.Cell death discovery · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While live 3D high resolution microscopy techniques are developing rapidly, their use for biological applications is partially hampered by practical difficulties such as the lack of a versatile sample chamber. Here, we propose the design of a multi-usage observation chamber adapted for live 3D bio-imaging. We show the usefulness and practicality of this chamber, which we named the UniverSlide, for live imaging of two case examples, namely multicellular systems encapsulated in sub-millimeter hydrogel shells and zebrafish larvae. We also demonstrate its versatility and compatibility with all microscopy devices by using upright or inverted microscope configurations after loading the UniverSlide with fixed or living samples. Further, the device is applicable for medium/high throughput screening and automatized multi-position image acquisition, providing a constraint-free but stable and parallelized immobilization of the samples. The frame of the UniverSlide is fabricated using a stereolithography 3D printer, has the size of a microscopy slide, is autoclavable and sealed with a removable lid, which makes it suitable for use in a controlled culture environment. We describe in details how to build this chamber and we provide all the files necessary to print the different pieces in the lab.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.