Evidence map›Paper›PMID 28186176›Full record

ArticleScientific reports2017

Dual Immunomagnetic Nanobeads-Based Lateral Flow Test Strip for Simultaneous Quantitative Detection of Carcinoembryonic Antigen and Neuron Specific Enolase.

Wenting Lu, Kan Wang, Kun Xiao, Weijian Qin, Yafei Hou, Hao Xu, Xinyu Yan, Yanrong Chen, Daxiang Cui, Jinghua He

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Review
  12. Magnetic Lateral Flow Immunoassays.Diagnostics (Basel, Switzerland) · 2020
    Review
  13. Article
  14. Developing Gold Nanoparticles-Conjugated Aflatoxin B1 Antifungal Strips.International journal of molecular sciences · 2019
    Article
  15. Review
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Wenting LuOutpatient Department, Zhujiang Hospital, Southern Medical University, 253 Gongye Road, Guangzhou, Guangdong 510280, China.
Kan WangSchool of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Kun XiaoSchool of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Weijian QinSchool of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Yafei HouSchool of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Hao XuSchool of Naval Architecture, Ocean &Civil Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Xinyu YanOutpatient Department, Zhujiang Hospital, Southern Medical University, 253 Gongye Road, Guangzhou, Guangdong 510280, China.
Yanrong ChenOutpatient Department, Zhujiang Hospital, Southern Medical University, 253 Gongye Road, Guangzhou, Guangdong 510280, China.
Daxiang CuiSchool of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
Jinghua HeOutpatient Department, Zhujiang Hospital, Southern Medical University, 253 Gongye Road, Guangzhou, Guangdong 510280, China.

Funding

Developmental Pathways to Substance Use Across European & Asian American YouthF31AA020700 · NIAAA · UNIVERSITY OF WASHINGTON · PI LUK, JEREMY WING-HEI · 2011 to 2013
$123k
NIAAA NIH HHS F31 AA020700
6 · The paper itself

Abstract

A novel immunomagnetic nanobeads -based lateral flow test strip was developed for the simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA), which are sensitive and specific in the clinical diagnosis of small cell lung cancer. Using this nanoscale method, high saturation magnetization, carboxyl-modified magnetic nanobeads were successfully synthesized. To obtain the immunomagnetic probes, a covalent bioconjugation of the magnetic nanobeads with the antibody of NSE and CEA was carried out. The detection area contained test line 1 and test line 2 which captured the immune complexes sensitively and formed sandwich complexes. In this assay, cross-reactivity results were negative and both NSE and CEA were detected simultaneously with no obvious influence on each other. The magnetic signal intensity of the nitrocellulose membrane was measured by a magnetic assay reader. For quantitative analysis, the calculated limit of detection was 0.094 ng/mL for NSE and 0.045 ng/mL for CEA. One hundred thirty clinical samples were used to validate the test strip which exhibited high sensitivity and specificity. This dual lateral flow test strip not only provided an easy, rapid, simultaneous quantitative detection strategy for NSE and CEA, but may also be valuable in automated and portable diagnostic applications.

Indexed as

Biomarkers, TumorCarcinoembryonic AntigenCross ReactionsHumansImmunomagnetic SeparationLung NeoplasmsNanostructuresPhosphopyruvate HydrataseReagent StripsSensitivity and SpecificitySmall Cell Lung CarcinomaBiomarkers, TumorCarcinoembryonic AntigenPhosphopyruvate HydrataseReagent Strips

Identifiers

PMID28186176
PMCPMC5301198

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.