ArticleScientific reports2017
Functionalized Graphene Oxide with Chitosan for Protein Nanocarriers to Protect against Enzymatic Cleavage and Retain Collagenase Activity.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 159 citations in OpenAlex.
- Article
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- Impact of chitosan supplementation on metabolomic profiles and microbial community dynamics in total mixed ration silage and rumen fluid.Animal bioscience · 2026Article
- Carbon-based nanomaterials in biomedicine and technology.Frontiers in chemistry · 2026Review
- An Overview of Biopolymer-Based Graphene Nanocomposites for Biotechnological Applications.Materials (Basel, Switzerland) · 2025Review
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- Article
- Enhanced efficacy of breast cancer treatment with etoposide-graphene oxide nanogels: A novel nanomedicine approach.BioImpacts : BI · 2025Article
- Anticancer activity of lactoferrin-coated biosynthesized selenium nanoparticles for combating different human cancer cells via mediating apoptotic effects.Scientific reports · 2023Article
- Advanced Theranostic Strategies for Viral Hepatitis Using Carbon Nanostructures.Micromachines · 2023Review
- Progress and challenges of graphene and its congeners for biomedical applications.Journal of molecular liquids · 2022Article
- Understanding the Role of the Lateral Dimensional Property of Graphene Oxide on Its Interactions with Renal Cells.Molecules (Basel, Switzerland) · 2022Article
- Augmenting apoptosis-mediated anticancer activity of lactoperoxidase and lactoferrin by nanocombination with copper and iron hybrid nanometals.Scientific reports · 2022Article
- Toward In-Field Determination of Nitrate Concentrations Via Diffusive Gradients in Thin Films-Incorporation of Reductants and Color Reagents.ACS omega · 2022Article
- Graphene Oxide Thin Films with Drug Delivery Function.Nanomaterials (Basel, Switzerland) · 2022Review
- Review
- Efficacy of a Graphene Oxide/Chitosan Sponge for Removal of Radioactive Iodine-131 from Aqueous Solutions.Life (Basel, Switzerland) · 2021Article
- Construction and Application of Graphene Oxide-Bovine Serum Albumin Modified Extended Gate Field Effect Transistor Chiral Sensor.Sensors (Basel, Switzerland) · 2021Article
- Design of graphenic nanocomposites containing chitosan and polyethylene glycol for spinal cord injury improvement.RSC advances · 2021Article
- Synthesis and Toxicity of Graphene Oxide Nanoparticles: A Literature Review ofBioMed research international · 2021Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteins have short half-life because of enzymatic cleavage. Here, a new protein nanocarrier made of graphene oxide (GO) + Chitosan (CS) is proposed to successfully prevent proteolysis in protein and simultaneously retain its activity. Bovine serum albumin (BSA) and collagenase were loaded on GO and GO-CS to explore the stability and activity of proteins. SEM, AFM, TEM, DSC, UV-Vis, FT-IR, RBS, Raman, SDS-PAGE and zymography were utilized as characterization techniques. The protecting role of GO and GO-CS against enzymatic cleavage was probed by protease digestion analysis on BSA, where the protease solution was introduced to GO-BSA and GO-CS-BSA at 37 °C for 0.5-1-3-6 hours. Characterizations showed the successful synthesis of few layers of GO and the coverage by CS. According to gelatin zymographic analysis, the loaded collagenase on GO and GO-CS lysed the gelatin and created non-staining bands which confirmed the activity of loaded collagenase. SDS-PAGE analysis revealed no significant change in the intact protein in the GO-BSA and GO-CS-BSA solution after 30-minute and 1-hour exposure to protease; however, free BSA was completely digested after 1 hour. After 6 hours, intact proteins were detected in GO-BSA and GO-CS-BSA solutions, while no intact protein was detected in the free BSA solution.
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