ArticleScientific reports2017
The first complete genome sequences of the acI lineage, the most abundant freshwater Actinobacteria, obtained by whole-genome-amplification of dilution-to-extinction cultures.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 34 papers.
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34 citing papers in PubMed.
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- The Saint-Leonard Urban Glaciotectonic Cave Harbors Rich and Diverse Planktonic and Sedimentary Microbial Communities.Microorganisms · 2024Article
- Ecological Trait-Based Digital Categorization of Microbial Genomes for Denitrification Potential.Microorganisms · 2024Article
- Flexible genomic island conservation across freshwater and marine Methylophilaceae.The ISME journal · 2024Article
- Isolation of phages infecting the abundant freshwater Actinobacteriota order 'Ca. Nanopelagicales'.The ISME journal · 2023Article
- Heliorhodopsin binds and regulates glutamine synthetase activity.PLoS biology · 2022Article
- First insights into the prokaryotic community structure of Lake Cote, Costa Rica: Influence on nutrient cycling.Frontiers in microbiology · 2022Article
- Heme auxotrophy in abundant aquatic microbial lineages.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Cultivation of Dominant Freshwater Bacterioplankton Lineages Using a High-Throughput Dilution-to-Extinction Culturing Approach Over a 1-Year Period.Frontiers in microbiology · 2021Article
- Assembly of Natively Synthesized Dual Chromophores Into Functional Actinorhodopsin.Frontiers in microbiology · 2021Article
- Crossing Treeline: Bacterioplankton Communities of Alpine and Subalpine Rocky Mountain Lakes.Frontiers in microbiology · 2021Article
- A Genomic Perspective Across Earth's Microbiomes Reveals That Genome Size in Archaea and Bacteria Is Linked to Ecosystem Type and Trophic Strategy.Frontiers in microbiology · 2021Review
- High-throughput cultivation based on dilution-to-extinction with catalase supplementation and a case study of cultivating acI bacteria from Lake Soyang.Journal of microbiology (Seoul, Korea) · 2020Article
- Microbial communities of the Laurentian Great Lakes reflect connectivity and local biogeochemistry.Environmental microbiology · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The acI lineage of the phylum Actinobacteria is the most abundant bacterial group in most freshwater lakes. However, due to difficulties in laboratory cultivation, only two mixed cultures and some incomplete single-amplified or metagenome-derived genomes have been reported for the lineage. Here, we report the initial cultivation and complete genome sequences of four novel strains of the acI lineage from the tribes acI-A1, -A4, -A7, and -C1. The acI strains, initially isolated by dilution-to-extinction culturing, eventually failed to be maintained as axenic cultures. However, the first complete genomes of the acI lineage were successfully obtained from these initial cultures through whole genome amplification applied to more than hundreds of cultured acI cells. The genome sequences exhibited features of genome streamlining and showed that the strains are aerobic chemoheterotrophs sharing central metabolic pathways, with some differences among tribes that may underlie niche diversification within the acI lineage. Actinorhodopsin was found in all strains, but retinal biosynthesis was complete in only A1 and A4 tribes.
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