Evidence map›Paper›PMID 28184026›Full record

ArticleOncotarget2017

Identification of TMEM208 and PQLC2 as reference genes for normalizing mRNA expression in colorectal cancer treated with aspirin.

Yuanyuan Zhu, Chao Yang, Mingjiao Weng, Yan Zhang, Chunhui Yang, Yinji Jin, Weiwei Yang, Yan He, Yiqi Wu, Yuhua Zhang and 13 more

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oncotarget, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.2field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors at 3 institutions in 1 country.

Yuanyuan ZhuDepartment of Gastrointestinal Medical Oncology, The Affiliated Tumor Hospital of Harbin Medical University, Harbin, China.
Chao YangDepartment of Pathology, Harbin Medical University, Harbin, China.
Mingjiao WengDepartment of Pathology, Harbin Medical University, Harbin, China.
Yan ZhangDepartment of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, Harbin, China.
Chunhui YangDepartment of Sports Humanities, Harbin Sport University, Harbin, China.
Yinji JinDepartment of Pathology, Harbin Medical University, Harbin, China.
Weiwei YangDepartment of Pathology, Harbin Medical University, Harbin, China.
Yan HeDepartment of Pathology, Harbin Medical University, Harbin, China.
Yiqi WuDepartment of Pathology, Harbin Medical University, Harbin, China.
Yuhua ZhangDepartment of Pathology, Harbin Medical University, Harbin, China.
Guangyu WangDepartment of Gastrointestinal Medical Oncology, The Affiliated Tumor Hospital of Harbin Medical University, Harbin, China.
Riju James RajkumarEzakiel RedpathDepartment of Pathology, Harbin Medical University, Harbin, China.
Lei ZhangDepartment of Pathology, Harbin Medical University, Harbin, China.
Xiaoming JinDepartment of Pathology, Harbin Medical University, Harbin, China.
Ying LiuDepartment of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, Harbin, China.
Yuchun SunCenter of Digital Dentistry, Pecking University School and Hospital of Stomatology, Beijing, China.
Ning NingDepartment of Gastrointestinal Surgery, International Hospital of Pecking University, Beijing, China.
Yu QiaoDepartment of Histology and Embryology, Harbin Medical University, Harbin, China.
Fengmin ZhangDepartment of Microbiology, Harbin Medical University, Harbin, China.
Zhiwei LiDepartment of Gastrointestinal Medical Oncology, The Affiliated Tumor Hospital of Harbin Medical University, Harbin, China.
Tianzhen WangDepartment of Pathology, Harbin Medical University, Harbin, China.
Yanqiao ZhangDepartment of Gastrointestinal Medical Oncology, The Affiliated Tumor Hospital of Harbin Medical University, Harbin, China.
Xiaobo LiDepartment of Pathology, Harbin Medical University, Harbin, China.
Harbin Medical University · CNPeking University · CNStomatology Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Numerous evidences indicate that aspirin usage causes a significant reduction in colorectal cancer. However, the molecular mechanisms about aspirin preventing colon cancer are largely unknown. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is a most frequently used method to identify the target molecules regulated by certain compound. However, this method needs stable internal reference genes to analyze the expression change of the targets. In this study, the transcriptional stabilities of several traditional reference genes were evaluated in colon cancer cells treated with aspirin, and also, the suitable internal reference genes were screened by using a microarray and were further identified by using the geNorm and NormFinder softwares, and then were validated in more cell lines and xenografts. We have showed that three traditional internal reference genes, β-actin, GAPDH and α-tubulin, are not suitable for studying gene transcription in colon cancer cells treated with aspirin, and we have identified and validated TMEM208 and PQLC2 as the ideal internal reference genes for detecting the molecular targets of aspirin in colon cancer in vitro and in vivo. This study reveals stable internal reference genes for studying the target genes of aspirin in colon cancer, which will contribute to identify the molecular mechanism behind aspirin preventing colon cancer.

Indexed as

ActinsAmino Acid Transport Systems, BasicAnimalsAnti-Inflammatory Agents, Non-SteroidalApoptosisAspirinCell ProliferationColorectal NeoplasmsGene Expression ProfilingHumansMaleMembrane ProteinsMiceMice, NudeReal-Time Polymerase Chain ReactionReference StandardsActinsAmino Acid Transport Systems, BasicAnti-Inflammatory Agents, Non-SteroidalAspirinMembrane ProteinsRNA, MessengerSLC66A1 protein, humanTMEM208 protein, humanTubulinaspirincolorectal cancerreference gene

Identifiers

PMID28184026
PMCPMC5410260
OpenAlexW2586079345

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.