ArticleBMC infectious diseases2017
Rapid and sensitive real-time assay for the detection of respiratory syncytial virus using RT-SIBA®.
Article in BMC infectious diseases, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
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- Comparison of Different Reverse Transcriptase-Polymerase Chain Reaction-Based Methods for Wastewater Surveillance of SARS-CoV-2: Exploratory Study.JMIR public health and surveillance · 2024Article
- Respiratory syncytial virus: A review of current basic and clinical knowledge.Qatar medical journal · 2024Review
- Manually pressurized droplet digital PCR chip for rapid SARS-CoV-2 diagnostics.Biomicrofluidics · 2024Article
- Exosomal Composition, Biogenesis and Profiling Using Point-of-Care Diagnostics-Implications for Cardiovascular Disease.Frontiers in cell and developmental biology · 2022Review
- SARS-CoV-2 detection using reverse transcription strand invasion based amplification and a portable compact size instrument.Scientific reports · 2021Article
- Article
- Recent advances in the detection of respiratory virus infection in humans.Journal of medical virology · 2020Review
- Detection of human rhinoviruses by reverse transcription strand invasion based amplification method (RT-SIBA).Journal of virological methods · 2019Article
- Molecular Detection of Streptococcus pyogenes by Strand Invasion Based Amplification Assay.Molecular diagnosis & therapy · 2018Article
- Development and evaluation of a rapid nucleic acid amplification method to detect influenza A and B viruses in human respiratory specimens.Diagnostic microbiology and infectious disease · 2018Article
- Respiratory Syncytial Virus: Targeting the G Protein Provides a New Approach for an Old Problem.Journal of virology · 2018Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRespiratory syncytial virus (RSV) is one of the most common causes of respiratory tract infections among young children and the elderly. Timely and accurate diagnosis of respiratory tract infections improves patient care and minimizes unnecessary prescriptions of antibiotics. We sought to develop a rapid nucleic acid tests for the detection of RSV within minutes, while retaining the high sensitivity achieved with RT-PCR.
methodsWe developed and evaluated a reverse transcription isothermal nucleic acid amplification method, reverse transcription strand invasion based amplification (RT-SIBA), for the rapid detection of RSV.
resultsThe developed RT-SIBA assay showed good sensitivity by detecting as few as 10 copies of RSV RNA within 20 min compared with reverse transcription polymerase chain reaction, which took approximately 2 h. The performance of the RT-SIBA RSV assay was further investigated using nasopharyngeal swab specimens. The RT-SIBA assay had a sensitivity of 100% (25/25) and a specificity of 100% (15/15).
conclusionRT-SIBA did not require highly purified RNA for the rapid detection of RSV and was therefore compatible with rapid specimen processing methods. This reduces the complexity of specimen preparation and further shortens the total amount of time needed to detect RSV in clinical specimens. The developed RT-SIBA assay for RSV could be a useful tool for prompt management of this infection.
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