ArticleBMC genomics2017
Comparative proteomics analysis of teleost intermuscular bones and ribs provides insight into their development.
Article in BMC genomics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 26 citations in OpenAlex.
- Integrated Transcriptomic and Proteomic Analysis RevealsAnimals : an open access journal from MDPI · 2026Article
- Creation of intermuscular bone-free genetic mutants in grass carp and multiomics reveals molecular regulatory basis.Science China. Life sciences · 2026Article
- Identification of Growth-Related SNPs and Genes Through Genome-Wide Association Studies in Pseudobagrus ussuriensis.Marine biotechnology (New York, N.Y.) · 2026Article
- Molecular mechanisms of intermuscular bone development in Acrossocheilus wenchowensis.Journal of fish biology · 2026Article
- Article
- Integrated transcriptomic analysis reveals evolutionary and developmental characteristics of tendon ossification in teleost.BMC biology · 2024Article
- Article
- A Comparative Genomic and Transcriptional Survey Providing Novel Insights into Bone Morphogenetic Protein 2 (International journal of molecular sciences · 2019Article
- Development of Teleost Intermuscular Bones Undergoing Intramembranous Ossification Based on Histological-Transcriptomic-Proteomic Data.International journal of molecular sciences · 2019Article
- Vertebrate SLRP family evolution and the subfunctionalization of osteoglycin gene duplicates in teleost fish.BMC evolutionary biology · 2018Article
- Comparative salivary proteomics analysis of children with and without dental caries using the iTRAQ/MRM approach.Journal of translational medicine · 2018Article
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIntermuscular bones (IBs) and ribs both are a part of skeletal system in teleosts, but with different developing process. The chemical composition of fish IBs and ribs as well as the underlying mechanism about their development have not been investigated. In the present study, histological structures showed that one bone cavity containing osteoclasts were existed in ribs, but not in IBs of Megalobrama amblycephala. We constructed the first proteomics map for fish bones including IBs and ribs, and identified the differentially expressed proteins between IBs and ribs through iTRAQ LC-MS/MS proteomic analysis.
resultsThe proteins extracted from IBs and ribs at 1- to 2-year old M. amblycephala were quantified 2,342 proteins, with 1,451 proteins annotated with GO annotation in biological processes, molecular function and cellular component. A number of bone related proteins as well as pathways were identified in the study. A total of 93 and 154 differently expressed proteins were identified in comparison groups of 1-IB-vs-1-Rib and 2-IB-vs-2-Rib, which indicated the obvious differences of chemical composition between these two bone tissues. The two proteins (vitronectin b precursor and matrix metalloproteinase-2) related to osteoclasts differentiation were significantly up-regulated in ribs compared with IBs (P < 0.05), which was in accordance with the results from histological structures. In comparison groups of 1-IB-vs-2-IB and 1-Rib-vs-2-Rib, 33 and 51 differently expressed proteins were identified and the function annotation results showed that these proteins were involved in regulating bone development and differentiation. Subsequently, 11 and 13 candidate proteins in comparison group of 1-IB-vs-1-Rib and 1-IB-vs-2-IB related to bone development were validated by MRM assays.
conclusionsOur present study suggested the different key proteins involved in the composition of fish ribs and IBs as well as their growth development. These findings could provide important clues towards further understanding of fish skeletal system and the roles of proteins playing in regulating diverse biological processes in fish.
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