ArticlePloS one2017
Selection and evaluation of new reference genes for RT-qPCR analysis in Epinephelus akaara based on transcriptome data.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Article
- Dataset for selection of stable reference genes for accurate quantitative gene expression analysis in silvertip tetra (Data in brief · 2024Article
- Optimal Reference Genes for RT-qPCR Experiments in Hippocampus and Cortex of Rats Chronically Exposed to Excessive Fluoride.Biological trace element research · 2024Article
- Screening of reference genes in tiger puffer (Takifugu rubripes) across tissues and under different nutritional conditions.Fish physiology and biochemistry · 2021Article
- Article
- Identification of reliable reference genes for expression studies in the magnum of laying hens housed in cage and cage-free systems.Veterinary medicine and science · 2021Article
- Induction of Gonadal Development in Protogynous Grouper with Orally Delivered FSH DNA.Marine biotechnology (New York, N.Y.) · 2019Article
- Reference Gene Validation via RT-qPCR for Human iPSC-Derived Neural Stem Cells and Neural Progenitors.Molecular neurobiology · 2019Article
- Selection and Validation of Novel RT-qPCR Reference Genes under Hormonal Stimuli and in Different Tissues of Santalum album.Scientific reports · 2018Article
- Identification of reference genes for RT-qPCR in the Antarctic moss Sanionia uncinata under abiotic stress conditions.PloS one · 2018Article
- Selection and evaluation of reference genes for qRT-PCR analysis inPlant methods · 2018Article
- Cathepsin B & D and the Survival of Early Embryos in Red Spotted Grouper,Development & reproduction · 2017Article
- Identification of reference genes for qRT-PCR in granulosa cells of healthy women and polycystic ovarian syndrome patients.Scientific reports · 2017Article
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5 authors.
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Abstract
Groupers are an economically important fish species in world fishery markets. Because many studies using RT-qPCR have addressed gene expression in groupers, appropriate reference genes are required to obtain reliable and accurate results. In this study, the most suitable reference genes were identified from eleven candidate genes of one of the most valuable species, Epinephelus akaara, in a range of different experimental conditions. Using the software packages geNorm, NormFinder, BestKeeper and refFinder, three traditionally used reference genes, β-actin (β-ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and beta-2-microglobulin (B2M), were identified as not suitable for E. akaara gene expression studies, whereas two newly identified reference genes, conserved oligomeric Golgi complex subunit 5 (Cog5) and brefeldin a-inhibited guanine nucleotide-exchange protein 1 (ARFGEF1), could be universally applied under all the tested conditions. These data provide the foundation for more precise results in RT-qPCR studies of gene expression in E. akaara and other Epinephelus species.
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