ArticlePloS one2017
A standardized human embryoid body platform for the detection and analysis of teratogens.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Klf5-adjacent super-enhancer functions as a 3D genome structure-dependent transcriptional driver to safeguard ESC identity.Nature communications · 2025Article
- Developmental Toxicity Studies: The Path towards Humanized 3D Stem Cell-Based Models.International journal of molecular sciences · 2023Review
- Article
- Pluripotent stem cell assays: Modalities and applications for predictive developmental toxicity.Current research in toxicology · 2022Article
- High Content Image Analysis of Spatiotemporal Proliferation and Differentiation Patterns in 3D Embryoid Body Differentiation Model.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Modeling human embryo development with embryonic and extra-embryonic stem cells.Developmental biology · 2021Review
- Comparative assessment of toxic responses in 3D embryoid body differentiation model and mouse early embryos treated with 5-hydroxytryptophan.Archives of toxicology · 2021Article
- Methoxyacetic acid inhibits histone deacetylase and impairs axial elongation morphogenesis of mouse gastruloids in a retinoic acid signaling-dependent manner.Birth defects research · 2020Article
- Exposure-based assessment of chemical teratogenicity using morphogenetic aggregates of human embryonic stem cells.Reproductive toxicology (Elmsford, N.Y.) · 2020Article
- Integrated Microphysiological Systems: Transferable Organ Models and Recirculating Flow.Advanced biosystems · 2019Review
- Teratogen screening with human pluripotent stem cells.Integrative biology : quantitative biosciences from nano to macro · 2018Review
- Pluripotent Stem Cells in Developmental Toxicity Testing: A Review of Methodological Advances.Toxicological sciences : an official journal of the Society of Toxicology · 2018Review
- Confocal micrographs: automated segmentation and quantitative shape analysis of neuronal cells treated with ostreolysin A/pleurotolysin B pore-forming complex.Histochemistry and cell biology · 2018Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Teratogens are compounds that can induce birth defects upon exposure of the developing fetus. To date, most teratogen studies utilize pregnant rodents to determine compound teratogenicity in vivo. However, this is a low throughput approach that cannot easily meet the need for comprehensive high-volume teratogen assessment, a goal of the US Environmental Protection Agency. In addition, rodent and human development differ substantially, and therefore the use of assays using relevant human cells has utility. For these reasons, interest has recently focused on the use of human embryonic stem cells for teratogen assessment. Here we present a highly standardized and quantitative system for the detection and analysis of teratogens that utilizes well-characterized and purified highly pluripotent stem cells. We have devised strategies to mass-produce thousands of uniformly sized spheroids of human ESCs (hESCs) that can be caused to undergo synchronous differentiation to yield embryoid bodies (EBs) in the presence and absence of suspected teratogens. The system uses all human cells and rigorously controlled and standardized EB culture conditions. Furthermore, the approach has been made quantitative by using high-content imaging approaches. Our system offers distinct advantages over earlier EB systems that rely heavily on the use on mouse ESCs and EB aggregates of stochastic sizes. Together, our results show that thousands of suspected teratogens could be assessed using human EB-based approaches.
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Registered trials
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