ArticlePloS one2017
Deciphering the molecular mechanism responsible for GCaMP6m's Ca2+-dependent change in fluorescence.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed, 136 citations in OpenAlex.
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- High-performance genetically-encoded green and red fluorescent biosensors for pyruvate.bioRxiv : the preprint server for biology · 2025Article
- Sensitive red fluorescent indicators for real-time visualization of potassium ion dynamics in vivo.PLoS biology · 2025Article
- Alcohol drinking is associated with greater calcium activity in mouse central amygdala dynorphin-expressing neurons.Progress in neuro-psychopharmacology & biological psychiatry · 2025Article
- Precise calcium-to-spike inference using biophysical generative models.Research square · 2025Article
- Obtaining artifact-corrected signals in fiber photometry via isosbestic signals, robust regression, and dF/F calculations.Neurophotonics · 2025Article
- NitrOFF: An engineered fluorescent biosensor to illuminate nitrate transport in living cells.bioRxiv : the preprint server for biology · 2025Article
- State-dependent motion of a genetically encoded fluorescent biosensor.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Chemigenetic Ca2+ indicators report elevated Ca2+ levels in endothelial Weibel-Palade bodies.PloS one · 2025Article
- Self-Assembly of Microstructured Protein Coatings with Programmable Functionality for Fluorescent Biosensors.ACS applied materials & interfaces · 2024Article
- Fluorescent Protein-Based Sensors for Detecting Essential Metal Ions across the Tree of Life.ACS sensors · 2024Review
- Removing crosstalk signals in neuron activity by time multiplexed excitations in a two-photon all-optical physiology system.Biomedical optics express · 2024Article
- Machine learning-guided engineering of genetically encoded fluorescent calcium indicators.Nature computational science · 2024Article
16 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The goal of this work is to determine how GCaMP6m's fluorescence is altered in response to Ca2+-binding. Our detailed spectroscopic study reveals the simplest explanation for how GCaMP6m changes fluorescence in response to Ca2+ is with a four-state model, in which a Ca2+-dependent change of the chromophore protonation state, due to a shift in pKa, is the predominant factor. The pKa shift is quantitatively explained by a change in electrostatic potential around the chromophore due to the conformational changes that occur in the protein when calmodulin binds Ca2+ and interacts with the M13 peptide. The absolute pKa values for the Ca2+-free and Ca2+-saturated states of GCaMP6m are critical to its high signal-to-noise ratio. This mechanism has important implications for further improvements to GCaMP6m and potentially for other similarly designed biosensors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.