ArticleScientific reports2017
High-throughput single-cell analysis for the proteomic dynamics study of the yeast osmotic stress response.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed.
- Quantitative Characterization of Gene Regulatory Circuits Associated With Fungal Secondary Metabolism to Discover Novel Natural Products.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- The regulatory mechanism of the yeast osmoresponse under different glucose concentrations.iScience · 2023Article
- Yeast cell fate control by temporal redundancy modulation of transcription factor paralogs.Nature communications · 2021Article
- Metabolic cost of rapid adaptation of single yeast cells.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Genome-scale transcriptional dynamics and environmental biosensing.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Protein and Proteome Measurements with Microfluidic Chips.Analytical chemistry · 2020Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Motorized fluorescence microscopy combined with high-throughput microfluidic chips is a powerful method to obtain information about different biological processes in cell biology studies. Generally, to observe different strains under different environments, high-throughput microfluidic chips require complex preparatory work. In this study, we designed a novel and easily operated high-throughput microfluidic system to observe 96 different GFP-tagged yeast strains in one switchable culture condition or 24 different GFP-tagged yeast strains in four parallel switchable culture conditions. A multi-pipette is the only additional equipment required for high-throughput patterning of cells in the chip. Only eight connections are needed to control 96 conditions. Using these devices, the proteomic dynamics of the yeast stress response pathway were carefully studied based on single-cell data. A new method to characterize the proteomic dynamics using a single cell's data is proposed and compared to previous methods, and the new technique should be useful for studying underlying control networks. Our method provides an easy and systematic way to study signaling pathways at the single-cell level.
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Registered trials
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