Evidence map›Paper›PMID 28179546›Full record

ArticleJournal of the Royal Society, Interface2017

Measurement of oxygen tension within mesenchymal stem cell spheroids.

Kaitlin C Murphy, Ben P Hung, Stephen Browne-Bourne, Dejie Zhou, Jessica Yeung, Damian C Genetos, J Kent Leach

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of the Royal Society, Interface, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.

0numbers the graph read from it
0cells of the map it votes in
80citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

80 citing papers in PubMed.

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20 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Kaitlin C MurphyDepartment of Biomedical Engineering, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Ben P HungDepartment of Biomedical Engineering, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Stephen Browne-BourneDepartment of Biomedical Engineering, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Dejie ZhouDepartment of Biomedical Engineering, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Jessica YeungDepartment of Biomedical Engineering, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
Damian C GenetosDepartment of Anatomy, Physiology, and Cell Biology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
J Kent LeachDepartment of Biomedical Engineering, School of Veterinary Medicine, University of California, Davis, CA 95616, USA jkleach@ucdavis.edu.ORCID 0000-0002-1673-3335

Funding

Biomaterial regulation of cell spheroids to synergistically enhance bone healingR01DE025475 · NIDCR · UNIVERSITY OF CALIFORNIA AT DAVIS · PI LEACH, J. KENT · 2015 to 2019
$1.9M
NIDCR NIH HHS R01 DE025475
6 · The paper itself

Abstract

Spheroids formed of mesenchymal stem cells (MSCs) exhibit increased cell survival and trophic factor secretion compared with dissociated MSCs, making them therapeutically advantageous for cell therapy. Presently, there is no consensus for the mechanism of action. Many hypothesize that spheroid formation potentiates cell function by generating a hypoxic core within spheroids of sufficiently large diameters. The purpose of this study was to experimentally determine whether a hypoxic core is generated in MSC spheroids by measuring oxygen tension in aggregates of increasing diameter and correlating oxygen tension values with cell function. MSC spheroids were formed with 15 000, 30 000 or 60 000 cells per spheroid, resulting in radii of 176 ± 8 µm, 251 ± 12 µm and 353 ± 18 µm, respectively. Oxygen tension values coupled with mathematical modelling revealed a gradient that varied less than 10% from the outer diameter within the largest spheroids. Despite the modest radial variance in oxygen tension, cellular metabolism from spheroids significantly decreased as the number of cells and resultant spheroid size increased. This may be due to adaptive reductions in matrix deposition and packing density with increases in spheroid diameter, enabling spheroids to avoid the formation of a hypoxic core. Overall, these data provide evidence that the enhanced function of MSC spheroids is not oxygen mediated.

Indexed as

HumansMesenchymal Stem CellsOxygenSpheroids, CellularOxygendiffusionhypoxiamesenchymal stem celloxygen tensionspheroid

Identifiers

PMID28179546
PMCPMC5332570

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.