ArticleJournal of virology2017
A Point Mutation in the RNA-Binding Domain of Human Parainfluenza Virus Type 2 Nucleoprotein Elicits Abnormally Enhanced Polymerase Activity.
Article in Journal of virology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 23 citations in OpenAlex.
- Open and closed forms of assembled henipavirus nucleoprotein suggest structural basis of genome access.Science advances · 2026Article
- Characterization of human parainfluenza virus type 2 circulating in southern France between 2017 and 2022 using whole-genome sequencing.Virus genes · 2026Article
- Article
- Novel transcription and replication-competent virus-like particles system modelling the Nipah virus life cycle.Emerging microbes & infections · 2024Article
- Nairovirus polymerase mutations associated with the establishment of persistent infection in human cells.Journal of virology · 2024Article
- Isolation, genome analysis and comparison of a novel parainfluenza virus 5 from a Siberian tiger (Frontiers in veterinary science · 2024Article
- Negative and ambisense RNA virus ribonucleocapsids: more than protective armor.Microbiology and molecular biology reviews : MMBR · 2023Review
- The bipartite promoter ofJournal of virology · 2023Article
- A Point Mutation in the Human Parainfluenza Virus Type 2 Nucleoprotein Leads to Two Separate Effects on Virus Replication.Journal of virology · 2022Article
- Review
- The Nucleocapsid of Paramyxoviruses: Structure and Function of an Encapsidated Template.Viruses · 2021Review
- A paramyxovirus-like model for Ebola virus bipartite promoters.PLoS pathogens · 2020Article
- Hexamer phasing governs transcription initiation in the 3'-leader of Ebola virus.RNA (New York, N.Y.) · 2020Article
- A Minigenome Study of Hazara Nairovirus Genomic Promoters.Journal of virology · 2019Article
- Assembly and cryo-EM structures of RNA-specific measles virus nucleocapsids provide mechanistic insight into paramyxoviral replication.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Bipartite promoters and RNA editing of paramyxoviruses and filoviruses.RNA (New York, N.Y.) · 2019Review
- The Nucleoprotein and Phosphoprotein of Measles Virus.Frontiers in microbiology · 2019Review
- The control of paramyxovirus genome hexamer length and mRNA editing.RNA (New York, N.Y.) · 2018Article
- Structure of the Paramyxovirus Parainfluenza Virus 5 Nucleoprotein in Complex with an Amino-Terminal Peptide of the Phosphoprotein.Journal of virology · 2018Article
- Dual function of the nuclear export signal of the Borna disease virus nucleoprotein in nuclear export activity and binding to viral phosphoprotein.Virology journal · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genome RNA of human parainfluenza virus type 2 (hPIV2) that acts as the template for the polymerase complex is entirely encapsidated by the nucleoprotein (NP). Recently, the crystal structure of NP of PIV5, a virus closely related to hPIV2, was resolved in association with RNA. Ten amino acids that contact the bound RNA were identified and are strictly conserved between PIV5 and hPIV2 NP. Mutation of hPIV2 NP Q202 (which contacts a base rather than the RNA backbone) to various amino acids resulted in an over 30-fold increase of polymerase activity as evidenced by a minireplicon assay, even though the RNA-binding affinity was unaltered. Using various modified minireplicons, we found that the enhanced reporter gene expression could be accounted for by increased minigenome replication, whereas mRNA synthesis itself was not affected by Q202 mutation. Moreover, the enhanced activities were still observed in minigenomes partially lacking the leader sequence and which were not of hexamer genome length. Unexpectedly, recombinant hPIV2 possessing the NP Q202A mutation could not be recovered from cDNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.