ArticleMicrobial cell factories2017
SWITCH: a dynamic CRISPR tool for genome engineering and metabolic pathway control for cell factory construction in Saccharomyces cerevisiae.
Article in Microbial cell factories, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 72 citations in OpenAlex.
- Metarhizium anisopliae engineering mediated by a CRISPR/Cas9 recyclable system.Folia microbiologica · 2026Article
- A CRISPR Cas12a/Cpf1 strategy to facilitate robust multiplex gene editing in Aspergillus Niger.Fungal biology and biotechnology · 2025Article
- Knocking out the carboxyltransferase interactor 1 (CTI1) in Chlamydomonas boosted oil content by fivefold without affecting cell growth.Plant biotechnology journal · 2025Article
- Oligonucleotide-based CRISPR-Cas9 toolbox for efficient engineering of Komagataella phaffii.FEMS yeast research · 2024Article
- CRISPR-Cas Technology for Bioengineering Conventional and Non-Conventional Yeasts: Progress and New Challenges.International journal of molecular sciences · 2023Review
- CRI-SPA: a high-throughput method for systematic genetic editing of yeast libraries.Nucleic acids research · 2023Article
- Practical Approaches for the YeastInternational journal of molecular sciences · 2023Review
- Recent Advances in CRISPR-Cas Technologies for Synthetic Biology.Journal of microbiology (Seoul, Korea) · 2023Review
- A Mad7 System for Genetic Engineering of Filamentous Fungi.Journal of fungi (Basel, Switzerland) · 2022Article
- ACtivE: Assembly and CRISPR-TargetedACS synthetic biology · 2022Article
- A Versatile in Vivo DNA Assembly Toolbox for Fungal Strain Engineering.ACS synthetic biology · 2022Article
- A gene cluster in Ginkgo biloba encodes unique multifunctional cytochrome P450s that initiate ginkgolide biosynthesis.Nature communications · 2022Article
- Modulating DNA Repair Pathways to Diversify Genomic Alterations in Saccharomyces cerevisiae.Microbiology spectrum · 2022Article
- Applications of CRISPR/Cas gene-editing technology in yeast and fungi.Archives of microbiology · 2021Article
- Expansion of the Yeast Modular Cloning Toolkit for CRISPR-Based Applications, Genomic Integrations and Combinatorial Libraries.ACS synthetic biology · 2021Article
- CRISPRi-Guided Metabolic Flux Engineering for Enhanced Protopanaxadiol Production inInternational journal of molecular sciences · 2021Article
- Customized yeast cell factories for biopharmaceuticals: from cell engineering to process scale up.Microbial cell factories · 2021Review
- A CRISPR/Cas9 method facilitates efficient oligo-mediated gene editing in Debaryomyces hansenii.Synthetic biology (Oxford, England) · 2021Article
- Transforming yeast peroxisomes into microfactories for the efficient production of high-value isoprenoids.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A CRISPRi-dCas9 System for Archaea and Its Use To Examine Gene Function during Nitrogen Fixation by Methanosarcina acetivorans.Applied and environmental microbiology · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe yeast Saccharomyces cerevisiae is increasingly used as a cell factory. However, cell factory construction time is a major obstacle towards using yeast for bio-production. Hence, tools to speed up cell factory construction are desirable.
resultsIn this study, we have developed a new Cas9/dCas9 based system, SWITCH, which allows Saccharomyces cerevisiae strains to iteratively alternate between a genetic engineering state and a pathway control state. Since Cas9 induced recombination events are crucial for SWITCH efficiency, we first developed a technique TAPE, which we have successfully used to address protospacer efficiency. As proof of concept of the use of SWITCH in cell factory construction, we have exploited the genetic engineering state of a SWITCH strain to insert the five genes necessary for naringenin production. Next, the naringenin cell factory was switched to the pathway control state where production was optimized by downregulating an essential gene TSC13, hence, reducing formation of a byproduct.
conclusionsWe have successfully integrated two CRISPR tools, one for genetic engineering and one for pathway control, into one system and successfully used it for cell factory construction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.