ArticlePloS one2017
Identification of a vacuolar proton channel that triggers the bioluminescent flash in dinoflagellates.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Who cites it
24 citing papers in PubMed, 43 citations in OpenAlex.
- Chemical stimulation sustains bioluminescence of living light materials.Science advances · 2026Article
- Bioluminescence of the heterotrophic dinoflagellate Polykrikos kofoidii Chatton 1914 (Dinophyceae).Journal of phycology · 2026Article
- Unraveling a Receptor-Mediated Bioluminescence Signaling Pathway in Red Tide Algae.Journal of molecular biology · 2025Article
- Marine eukaryote bioluminescence: a review of species and their functional biology.Marine life science & technology · 2025Review
- Interaction with stomatin directs human proton channels into cholesterol-dependent membrane domains.Biophysical journal · 2024Article
- Transcendent Aspects of Proton Channels.Annual review of physiology · 2024Review
- Ion currents through the voltage sensor domain of distinct families of proteins.Journal of biological physics · 2023Review
- Voltage-Gated Proton Channels in the Tree of Life.Biomolecules · 2023Review
- Unexpected expansion of the voltage-gated proton channel family.The FEBS journal · 2023Article
- Bioluminescent Dinoflagellates as a Bioassay for Toxicity Assessment.International journal of molecular sciences · 2022Review
- Reduced HProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Voltage-gated proton channels in polyneopteran insects.FEBS open bio · 2022Article
- Analysis of an electrostatic mechanism for ΔpH dependent gating of the voltage-gated proton channel, HBiochimica et biophysica acta. Bioenergetics · 2021Review
- Acquisition of bioluminescent trait by non-luminous organisms from luminous organisms through various origins.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2021Review
- Cryo-EM structures of the TTYH family reveal a novel architecture for lipid interactions.Nature communications · 2021Article
- Zinc modulation of proton currents in a new voltage-gated proton channel suggests a mechanism of inhibition.The FEBS journal · 2020Article
- Insights on small molecule binding to the Hv1 proton channel from free energy calculations with molecular dynamics simulations.Scientific reports · 2020Article
- New Perspectives Related to the Bioluminescent System in Dinoflagellates:International journal of molecular sciences · 2020Review
- Molecular and biochemical basis for the loss of bioluminescence in the dinoflagellateLimnology and oceanography · 2019Article
- Hydrophobic gasket mutation produces gating pore currents in closed human voltage-gated proton channels.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 1 country.
Funding
Abstract
In 1972, J. Woodland Hastings and colleagues predicted the existence of a proton selective channel (HV1) that opens in response to depolarizing voltage across the vacuole membrane of bioluminescent dinoflagellates and conducts protons into specialized luminescence compartments (scintillons), thereby causing a pH drop that triggers light emission. HV1 channels were subsequently identified and demonstrated to have important functions in a multitude of eukaryotic cells. Here we report a predicted protein from Lingulodinium polyedrum that displays hallmark properties of bona fide HV1, including time-dependent opening with depolarization, perfect proton selectivity, and characteristic ΔpH dependent gating. Western blotting and fluorescence confocal microscopy of isolated L. polyedrum scintillons immunostained with antibody to LpHV1 confirm LpHV1's predicted organellar location. Proteomics analysis demonstrates that isolated scintillon preparations contain peptides that map to LpHV1. Finally, Zn2+ inhibits both LpHV1 proton current and the acid-induced flash in isolated scintillons. These results implicate LpHV1 as the voltage gated proton channel that triggers bioluminescence in L. polyedrum, confirming Hastings' hypothesis. The same channel likely mediates the action potential that communicates the signal along the tonoplast to the scintillon.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.