ArticleMemorias do Instituto Oswaldo Cruz2017
Achievement of constitutive fluorescent pLEXSY-egfp Leishmania braziliensis and its application as an alternative method for drug screening in vitro.
Article in Memorias do Instituto Oswaldo Cruz, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Article
- Synthesis and Antileishmanial Evaluation ofACS omega · 2025Article
- Anti-Antimicrobial agents and chemotherapy · 2024Article
- Reporter gene systems: A powerful tool forCurrent research in microbial sciences · 2022Article
- Annatto Oil Loaded Nanostructured Lipid Carriers: A Potential New Treatment for Cutaneous Leishmaniasis.Pharmaceutics · 2021Article
- Ros3 (Lem3p/CDC50) Gene Dosage Is Implicated in Miltefosine Susceptibility inACS infectious diseases · 2021Article
- Article
- Determination of anti-leishmanial drugs efficacy againstParasite epidemiology and control · 2020Article
- Generation and Characterization of a Dual-Reporter TransgenicFrontiers in cellular and infection microbiology · 2019Article
- Biochemical analysis of leishmanial and human GDP-Mannose Pyrophosphorylases and selection of inhibitors as new leads.Scientific reports · 2017Article
- Parasite Burden Measurement in theIranian journal of parasitologyArticle
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7 authors.
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Abstract
backgroundGene reporter-fluorescent cells have emerged as alternative method for drug screening.
objectiveAchievement of constitutive expression of fluorescent protein GFP by Leishmania braziliensis as alternative method for drug screening.
methodsL. braziliensis-GFP was generated using Leishmania tarentolae pLEXSY-egfp for constitutive expression of GFP. Fluorescent cells were selected and subjected to standardisation tests of anti-promastigote and anti-intracellular amastigote assays.
findingsOur results showed that L. braziliensis-GFP method is faster and more sensitive than Allamar Blue-resazurin. MAIN
conclusionTransfected parasites maintained stable fluorescence after successive in vitro passages and pLEXSY system can be used to achieve non-L. tarentolae fluorescent cells.
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