Evidence map›Paper›PMID 28177043›Full record

ArticleMemorias do Instituto Oswaldo Cruz2017

Performance of a molecular assay to detect Mycobacterium tuberculosis complex DNA in clinical specimens: multicenter study in Brazil.

Mirela Verza, Karen Barros Schmid, Regina Bones Barcellos, Natali Linck, Graziele Lima Bello, Daniel Scapin, Rosa Dea Sperhacke, Márcia Susana Nunes Silva, Claudia Wollheim, Martha Gabriela Celle Rivero and 5 more

Abstract readEvaluation StudyMulticenter Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Memorias do Instituto Oswaldo Cruz, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Improvement of Mycobacterium tuberculosis detection in sputum using DNA extracted by sonication.The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Mirela VerzaCentro de Desenvolvimento Científico e Tecnológico, Fundação Estadual de Produção e Pesquisa em Saúde, Porto Alegre, RS, Brasil.
Karen Barros SchmidCentro de Desenvolvimento Científico e Tecnológico, Fundação Estadual de Produção e Pesquisa em Saúde, Porto Alegre, RS, Brasil.
Regina Bones BarcellosCentro de Desenvolvimento Científico e Tecnológico, Fundação Estadual de Produção e Pesquisa em Saúde, Porto Alegre, RS, Brasil.
Natali LinckUniversidade Luterana do Brasil, Canoas, RS, Brasil.
Graziele Lima BelloUniversidade Luterana do Brasil, Canoas, RS, Brasil.
Daniel ScapinUniversidade Luterana do Brasil, Canoas, RS, Brasil.
Rosa Dea SperhackeUniversidade de Caxias do Sul, Laboratório de Pesquisa em HIV/AIDS, Caxias do Sul, RS, Brasil.
Márcia Susana Nunes SilvaUniversidade Luterana do Brasil, Canoas, RS, Brasil.
Claudia WollheimUniversidade de Caxias do Sul, Laboratório de Microbiologia Clínica, Caxias do Sul, RS, Brasil.
Martha Gabriela Celle RiveroUniversidade Federal de São Paulo, Laboratório Especial de Microbiologia Clínica, São Paulo, SP, Brasil.
Afrânio Lineu KritskiUniversidade Federal do Rio de Janeiro, Faculdade de Medicina, Centro de Pesquisa em Tuberculose do Hospital Universitário Clementino Fraga Filho, Laboratório de Genética e Biologia Molecular, Rio de Janeiro, RJ, Brasil.
Leonides RezendeLabtest, Belo Horizonte, MG, Brasil.
Martha Maria OliveiraUniversidade Federal do Rio de Janeiro, Faculdade de Medicina, Centro de Pesquisa em Tuberculose do Hospital Universitário Clementino Fraga Filho, Laboratório de Genética e Biologia Molecular, Rio de Janeiro, RJ, Brasil.
Elis Regina Dalla CostaCentro de Desenvolvimento Científico e Tecnológico, Fundação Estadual de Produção e Pesquisa em Saúde, Porto Alegre, RS, Brasil.
Maria Lucia Rosa RossettiCentro de Desenvolvimento Científico e Tecnológico, Fundação Estadual de Produção e Pesquisa em Saúde, Porto Alegre, RS, Brasil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIn high tuberculosis (TB) burden countries, there are few data on the performance of new molecular commercialised assays developed locally.

objectiveTo evaluate the performance of a new molecular commercialised assay for TB diagnosis (Detect-TB) in three laboratories.

methodsA total of 302 sputum samples from an equal number of patients with presumptive diagnosis of pulmonary tuberculosis (PTB) were submitted for routine smear microscopy, culture, and Detect-TB assay at three different sites in Brazil (the cities of Caxias do Sul, São Paulo and Canoas).

findingsSeventy four (24.7%) TB cases were diagnosed (65 bacteriologically confirmed). When compared to smear microscopy/culture results, the overall sensitivity and specificity of Detect-TB assay was 84.6% (CI 95%; 73.7-91.6) and 93.1% (CI 95%; 89.1-95.8), respectively. When compared to bacteriological and clinical diagnostic criteria, the sensitivity and specificity of Detect-TB assay was 74.3% (CI 95%; 63.3-82.9) and 92.9% (CI 95%; 88.7-95.6), respectively. Among the three sites - Caxias do Sul, São Paulo and Canoas - the sensitivity and specificity were respectively 94.7% and 97.8%; 71.4% and 93.9%, 82.1% and 88.9%. MAIN

conclusionsThese findings suggest that the Detect-TB assay could be applied routinely in reference laboratories across different regions in Brazil.

Indexed as

BrazilDNA, BacterialFalse Negative ReactionsFalse Positive ReactionsHumansMycobacterium tuberculosisSensitivity and SpecificitySputumTuberculosis, PulmonaryDNA, Bacterial

Identifiers

PMID28177043
PMCPMC5293118

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.