ArticleScientific reports2017
A versatile assay to determine bacterial and host factors contributing to opsonophagocytotic killing in hirudin-anticoagulated whole blood.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 38 citations in OpenAlex.
- The Effect of a Multivitamin and Mineral Supplement on Immune Function in Healthy Older Adults: A Double-Blind, Randomized, Controlled Trial.Nutrients · 2020Trial
- Pathogen-specific kinetics of oxidative burst in camel leukocytes: Influence of serum opsonization on reactive oxygen species production.Veterinary world · 2025Article
- Age-related decline in IgM responses associate with reduced opsonophagocytic activity following PCV13 vaccination.NPJ vaccines · 2025Article
- The role of opsonophagocytosis in killing ofCurrent research in microbial sciences · 2025Article
- Article
- Local complement activation and modulation in mucosal immunity.Mucosal immunology · 2024Review
- Harnessing the combined effect of antivirulence agent trans-chalcone with bactericidal curcumin against sortase A enzyme to tackle Gram-positive bacterial infections.Folia microbiologica · 2024Article
- Novel antimicrobial peptides identified in legume plant,Microbiology spectrum · 2024Article
- Review
- Silencing EGFR-upregulated expression of CD55 and CD59 activates the complement system and sensitizes lung cancer to checkpoint blockade.Nature cancer · 2022Article
- Secondary Complement Deficiency Impairs Anti-Microbial Immunity toFrontiers in immunology · 2022Article
- Monoclonal antibodies effectively potentiate complement activation and phagocytosis ofFrontiers in immunology · 2022Article
- The Old Yellow Enzyme OfrA FostersFrontiers in microbiology · 2022Article
- The N2N3 domains of ClfA, FnbpA and FnbpB in Staphylococcus aureus bind to human complement factor H, and their antibodies enhance the bactericidal capability of human blood.Journal of biochemistry · 2021Article
- Resolving trained immunity with systems biology.European journal of immunology · 2021Review
- Article
- Alternative Complement Pathway Inhibition Abrogates Pneumococcal Opsonophagocytosis in Vaccine-Naïve, but Not in Vaccinated Individuals.Frontiers in immunology · 2021Article
- Bacterial behavior in human blood reveals complement evaders with some persister-like features.PLoS pathogens · 2020Article
- Eculizumab impairs Neisseria meningitidis serogroup B killing in whole blood despite 4CMenB vaccination of PNH patients.Blood advances · 2020Article
- Effect of Short-Term Antimicrobial Therapy on the Tolerance and Antibiotic Resistance of Multidrug-ResistantInfection and drug resistance · 2020Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most bacteria entering the bloodstream will be eliminated through complement activation on the bacterial surface and opsonophagocytosis. However, when these protective innate immune systems do not work optimally, or when bacteria are equipped with immune evasion mechanisms that prevent killing, this can lead to serious infections such as bacteremia and meningitis, which is associated with high morbidity and mortality. In order to study the complement evasion mechanisms of bacteria and the capacity of human blood to opsonize and kill bacteria, we developed a versatile whole blood killing assay wherein both phagocyte function and complement activity can easily be monitored and modulated. In this assay we use a selective thrombin inhibitor hirudin to fully preserve complement activity of whole blood. This assay allows controlled analysis of the requirements for active complement by replacing or heat-inactivating plasma, phagocyte function and bacterial immune evasion mechanisms that contribute to survival in human blood.
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