ArticleScientific reports2017
eUnaG: a new ligand-inducible fluorescent reporter to detect drug transporter activity in live cells.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Understanding how genetically encoded tags and crowding agents affect phase separation by heterochromatin protein HP1α.Cell reports methods · 2025Article
- The HELP-UnaG Fusion Protein as a Bilirubin Biosensor: From Theory to Mature Technological Development.Molecules (Basel, Switzerland) · 2025Review
- Exonuclease-enhanced prime editors.Nature methods · 2024Article
- Genetically encoded barcodes for correlative volume electron microscopy.Nature biotechnology · 2023Article
- Using Blue Mini-LEDs as a Light Source Designed a Miniaturized Optomechanical Device for the Detection of Direct Bilirubin.Nanoscale research letters · 2022Article
- Macromolecular and Solution Properties of the Recombinant Fusion Protein HUG.Biomacromolecules · 2022Article
- Identification and characterization of a heme exporter from the MRP family in Drosophila melanogaster.BMC biology · 2022Article
- Characterization of a novel pesticide transporter and P-glycoprotein orthologues inProceedings. Biological sciences · 2022Article
- A Protein-Engineered, Enhanced Yeast Display Platform for Rapid Evolution of Challenging Targets.ACS synthetic biology · 2021Article
- Enhanced UnaG With Minimal Labeling Artifact for Single-Molecule Localization Microscopy.Frontiers in molecular biosciences · 2021Article
- Imaging living obligate anaerobic bacteria with bilin-binding fluorescent proteins.Current research in microbial sciences · 2020Article
- Disrupting Fluorescence by Mutagenesis in a Green Fluorescent Fatty Acid Binding Protein from a Marine Eel.The protein journal · 2020Article
- Bright ligand-activatable fluorescent protein for high-quality multicolor live-cell super-resolution microscopy.Nature communications · 2020Article
- Fluorescence enhancement of a ligand-activated fluorescent protein induced by collective noncovalent interactions.Chemical science · 2018Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 3 countries.
Funding
Abstract
The absorption, distribution, metabolism and excretion (ADME) of metabolites and toxic organic solutes are orchestrated by the ATP-binding cassette (ABC) transporters and the organic solute carrier family (SLC) proteins. A large number of ABC and SLC transpoters exist; however, only a small number have been well characterized. To facilitate the analysis of these transporters, which is important for drug safety and physiological studies, we developed a sensitive genetically encoded bilirubin (BR)-inducible fluorescence sensor (eUnaG) to detect transporter-coupled influx/efflux of organic compounds. This sensor can be used in live cells to measure transporter activity, as excretion of BR depends on ABC and SLC transporters. Applying eUnaG in functional RNAi screens, we characterize l(2)03659 as a Drosophila multidrug resistant-associated ABC transporter.
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Registered trials
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