ArticleAMB Express2017
Effect of nitrogen availability on the poly-3-D-hydroxybutyrate accumulation by engineered Saccharomyces cerevisiae.
Article in AMB Express, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- EngineeringSynthetic and systems biotechnology · 2026Article
- PHA synthase variant design using a conditional variational autoencoder.PLoS computational biology · 2026Article
- Comparison of poly-3-hydroxybutyrate (P3HB) synthesis by Bacillus cereus and Azotobacter vinelandii OP: effect of agitation on the accumulation and physicochemical properties of the biopolymer.Bioresources and bioprocessing · 2025Article
- Synthetic biology strategies for sustainable bioplastic production by yeasts.Journal of microbiology (Seoul, Korea) · 2025Review
- Review
- Design and thermodynamic analysis of a pathway enabling anaerobic production of poly-3-hydroxybutyrate inSynthetic and systems biotechnology · 2023Article
- Using phosphoglucose isomerase-deficient (pgi1Δ) Saccharomyces cerevisiae to map the impact of sugar phosphate levels on D-glucose and D-xylose sensing.Microbial cell factories · 2022Article
- PHB production from cellobiose with Saccharomyces cerevisiae.Microbial cell factories · 2022Article
- Control of D-lactic acid content in P(LA-3HB) copolymer in the yeastMetabolic engineering communications · 2022Article
- Review
- Polyhydroxyalkanoate Production byMicroorganisms · 2022Article
- Production of D-lactic acid containing polyhydroxyalkanoate polymers in yeast Saccharomyces cerevisiae.Journal of industrial microbiology & biotechnology · 2021Article
- Emergent Approaches to Efficient and Sustainable Polyhydroxyalkanoate Production.Molecules (Basel, Switzerland) · 2021Review
- Haloarchaea as Cell Factories to Produce Bioplastics.Marine drugs · 2021Review
- Current Challenges and Opportunities in Non-native Chemical Production by Engineered Yeasts.Frontiers in bioengineering and biotechnology · 2020Review
- Growth kinetics, fatty acid composition and metabolic activity changes of Crypthecodinium cohnii under different nitrogen source and concentration.AMB Express · 2017Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Poly-3-D-hydroxybutyrate (or PHB) is a polyester which can be used in the production of biodegradable plastics from renewable resources. It is naturally produced by several bacteria as a response to nutrient starvation in the excess of a carbon source. The yeast Saccharomyces cerevisiae could be an alternative production host as it offers good inhibitor tolerance towards weak acids and phenolic compounds and does not depolymerize the produced PHB. As nitrogen limitation is known to boost the accumulation of PHB in bacteria, the present study aimed at investigating the effect of nitrogen availability on PHB accumulation in two recombinant S. cerevisiae strains harboring different xylose consuming and PHB producing pathways: TMB4443 expressing an NADPH-dependent acetoacetyl-CoA reductase and a wild-type S. stipitis XR with preferential use of NADPH and TMB4425 which expresses an NADH-dependent acetoacetyl-CoA reductase and a mutated XR with a balanced affinity for NADPH/NADH. TMB4443 accumulated most PHB under aerobic conditions and with glucose as sole carbon source, whereas the highest PHB concentrations were obtained with TMB4425 under anaerobic conditions and xylose as carbon source. In both cases, the highest PHB contents were obtained with high availability of nitrogen. The major impact of nitrogen availability was observed in TMB4425, where a 2.7-fold increase in PHB content was obtained. In contrast to what was observed in natural PHB-producing bacteria, nitrogen deficiency did not improve PHB accumulation in S. cerevisiae. Instead the excess available carbon from xylose was shunted into glycogen, indicating a significant gluconeogenic activity on xylose.
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