ArticleCurrent microbiology2017
False-Positive Viability PCR Results: An Association with Microtubes.
Article in Current microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Who cites it
14 citing papers in PubMed.
- Optimization of the viability PCR for accurate detection of Staphylococcus aureus in food samples.PloS one · 2025Article
- Subtracting the background by reducing cell-free DNA's confounding effects on Mycobacterium tuberculosis quantitation and the sputum microbiome.Scientific reports · 2024Article
- Asymmetric split-ring plasmonic nanostructures for the optical sensing ofBiomedical optics express · 2023Article
- An Improved Real-Time Viability PCR Assay to DetectInternational journal of molecular sciences · 2022Article
- Screening and identification of BP100 peptide conjugates active against Xylella fastidiosa using a viability-qPCR method.BMC microbiology · 2020Article
- Key Factors for Removing Bias in Viability PCR-Based Methods: A Review.Current microbiology · 2020Review
- Detection and Quantification Methods for Viable but Non-culturable (VBNC) Cells in Process Wash Water of Fresh-Cut Produce: Industrial Validation.Frontiers in microbiology · 2020Article
- Viability RT-qPCR Combined with Sodium Deoxycholate Pre-treatment for Selective Quantification of Infectious Viruses in Drinking Water Samples.Food and environmental virology · 2019Article
- Article
- Optimization of a Viability PCR Method for the Detection of Listeria monocytogenes in Food Samples.Current microbiology · 2018Article
- Monitoring Viable Cells of the Biological Control Agent Lactobacillus plantarum PM411 in Aerial Plant Surfaces by Means of a Strain-Specific Viability Quantitative PCR Method.Applied and environmental microbiology · 2018Article
- Response: Commentary: Reducing Viability Bias in Analysis of Gut Microbiota in Preterm Infants at Risk of NEC and Sepsis.Frontiers in cellular and infection microbiology · 2018Article
- Commentary: Reducing Viability Bias in Analysis of Gut Microbiota in Preterm Infants at Risk of NEC and Sepsis.Frontiers in cellular and infection microbiology · 2018Article
- Improved sample treatment protocol for accurate detection of live Salmonella spp. in food samples by viability PCR.PloS one · 2017Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Currently, one of the most challenged points to expand the use of viability PCR technique is achieving the complete exclusion of dead cells amplification signals, thus avoiding the overestimation of live cells population. Considering that, and based on the hypothesis that DNA may be retained by microtube walls, the impact of the microtube was addressed on signals from live and heat-killed cells. A double-dye reagent, PEMAX™, which comprises a mix of photo-reactive azide forms of phenanthridium, was used in this work. We found that if both the incubation and the photoactivation steps are carried out in different microtubes, the dead cell signal is greatly reduced than when those steps are done in the same tube. Therefore, the strategy depicted in this study presents a simple and efficient step in minimizing false-positive signal when employing viability PCR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.