Evidence map›Paper›PMID 28174601›Full record

ArticleBiotechnology for biofuels2017

Development of a genetically programed vanillin-sensing bacterium for high-throughput screening of lignin-degrading enzyme libraries.

Barindra Sana, Kuan Hui Burton Chia, Sarada S Raghavan, Balamurugan Ramalingam, Niranjan Nagarajan, Jayasree Seayad, Farid J Ghadessy

Open access · goldAbstract read
In one paragraph

Article in Biotechnology for biofuels, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
3.3field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 35 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
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  6. Review
  7. Article
  8. Review
  9. Review
  10. Sourcing VanillinFood technology and biotechnology
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Barindra Sanap53 Laboratory, Agency for Science Technology And Research (ASTAR), 8A Biomedical Grove, #06-04/05 Neuros/Immunos, Singapore, 138648 Singapore.
Kuan Hui Burton ChiaGenome Institute of Singapore, 60 Biopolis Street, Genome, #02-01, Singapore, 138672 Singapore.
Sarada S Raghavanp53 Laboratory, Agency for Science Technology And Research (ASTAR), 8A Biomedical Grove, #06-04/05 Neuros/Immunos, Singapore, 138648 Singapore.
Balamurugan RamalingamInstitute of Chemical and Engineering Sciences, 8 Biomedical Grove, Neuros, #07-01, Singapore, 138665 Singapore.
Niranjan NagarajanGenome Institute of Singapore, 60 Biopolis Street, Genome, #02-01, Singapore, 138672 Singapore.
Jayasree SeayadInstitute of Chemical and Engineering Sciences, 8 Biomedical Grove, Neuros, #07-01, Singapore, 138665 Singapore.
Farid J Ghadessyp53 Laboratory, Agency for Science Technology And Research (ASTAR), 8A Biomedical Grove, #06-04/05 Neuros/Immunos, Singapore, 138648 Singapore.
Agency for Science, Technology and Research · SGGenome Institute of Singapore · SGInstitute of Chemical and Engineering Sciences · SG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundLignin is a potential biorefinery feedstock for the production of value-added chemicals including vanillin. A huge amount of lignin is produced as a by-product of the paper industry, while cellulosic components of plant biomass are utilized for the production of paper pulp. In spite of vast potential, lignin remains the least exploited component of plant biomass due to its extremely complex and heterogenous structure. Several enzymes have been reported to have lignin-degrading properties and could be potentially used in lignin biorefining if their catalytic properties could be improved by enzyme engineering. The much needed improvement of lignin-degrading enzymes by high-throughput selection techniques such as directed evolution is currently limited, as robust methods for detecting the conversion of lignin to desired small molecules are not available.

resultsWe identified a vanillin-inducible promoter by RNAseq analysis of

conclusionsWe developed an engineered

Indexed as

BiorefineryDirected evolutionEnzyme engineeringFluorescence-activated cell sortingHigh-throughput screeningLigninMicrobial biosensorRNA sequencingVanillinVanillin-inducible promoter

Identifiers

PMID28174601
PMCPMC5291986
OpenAlexW2584936337

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.