ArticleProceedings of the National Academy of Sciences of the United States of America2017
Reelin transiently promotes N-cadherin-dependent neuronal adhesion during mouse cortical development.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 58 citations in OpenAlex.
- Accomplishments of "Old-Fashioned" Electron Microscopy in the Period of Dominance of Immunofluorescent Methods.International journal of molecular sciences · 2026Review
- A new paradigm considering multicellular adhesion, repulsion and attraction represent diverse cellular tile patterns.PLoS computational biology · 2025Article
- De novo monoallelic Reelin missense variants cause dominant neuronal migration disorders via a dominant-negative mechanism.The Journal of clinical investigation · 2024Article
- A Case of Anorexia Nervosa with Focal Cortical Dysplasia.Case reports in psychiatry · 2024Article
- The embryonic patterning gene Dbx1 governs the survival of the auditory midbrain via Tcf7l2-Ap2δ transcriptional cascade.Cell death and differentiation · 2023Article
- HeterozygouseNeuro · 2023Article
- Molecular mechanisms of corpus callosum development: a four-step journey.Frontiers in neuroanatomy · 2023Review
- CBP-HSF2 structural and functional interplay in Rubinstein-Taybi neurodevelopmental disorder.Nature communications · 2022Article
- Development of intensiometric indicators for visualizing N-cadherin interaction across cells.Communications biology · 2022Article
- Specific contribution of Reelin expressed by Cajal-Retzius cells or GABAergic interneurons to cortical lamination.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Phosphorylation of Focal Adhesion Kinase at Y925: Role in Glia-Dependent and Independent Migration through Regulating Cofilin and N-Cadherin.Molecular neurobiology · 2022Article
- Flying under the radar: CDH2 (N-cadherin), an important hub molecule in neurodevelopmental and neurodegenerative diseases.Frontiers in neuroscience · 2022Review
- Regulation of Cell Delamination During Cortical Neurodevelopment and Implication for Brain Disorders.Frontiers in neuroscience · 2022Review
- X-chromosome variants are associated with aldosterone producing adenomas.Scientific reports · 2021Article
- Poly (ADP-Ribose) Polymerase 1 Regulates Cajal-Retzius Cell Development and Neural Precursor Cell Adhesion.Frontiers in cell and developmental biology · 2021Article
- p73 as a Tissue Architect.Frontiers in cell and developmental biology · 2021Review
- Development of an N-Cadherin Biofunctionalized Hydrogel to Support the Formation of Synaptically Connected Neural Networks.ACS biomaterials science & engineering · 2020Article
- Mathematical models for cell migration: a non-local perspective.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Reelin Improves Cognition and Extends the Lifespan of Mutant Ndel1 Mice with Postnatal CA1 Hippocampus Deterioration.Cerebral cortex (New York, N.Y. : 1991) · 2020Article
- How Do Cortical Excitatory Neurons Terminate Their Migration at the Right Place? Critical Roles of Environmental Elements.Frontiers in cell and developmental biology · 2020Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reelin is an essential glycoprotein for the establishment of the highly organized six-layered structure of neurons of the mammalian neocortex. Although the role of Reelin in the control of neuronal migration has been extensively studied at the molecular level, the mechanisms underlying Reelin-dependent neuronal layer organization are not yet fully understood. In this study, we directly showed that Reelin promotes adhesion among dissociated neocortical neurons in culture. The Reelin-mediated neuronal aggregation occurs in an N-cadherin-dependent manner, both in vivo and in vitro. Unexpectedly, however, in a rotation culture of dissociated neocortical cells that gradually reaggregated over time, we found that it was the neural progenitor cells [radial glial cells (RGCs)], rather than the neurons, that tended to form clusters in the presence of Reelin. Mathematical modeling suggested that this clustering of RGCs could be recapitulated if the Reelin-dependent promotion of neuronal adhesion were to occur only transiently. Thus, we directly measured the adhesive force between neurons and N-cadherin by atomic force microscopy, and found that Reelin indeed enhanced the adhesiveness of neurons to N-cadherin; this enhanced adhesiveness began to be observed at 30 min after Reelin stimulation, but declined by 3 h. These results suggest that Reelin transiently (and not persistently) promotes N-cadherin-mediated neuronal aggregation. When N-cadherin and stabilized β-catenin were overexpressed in the migrating neurons, the transfected neurons were abnormally distributed in the superficial region of the neocortex, suggesting that appropriate regulation of N-cadherin-mediated adhesion is important for correct positioning of the neurons during neocortical development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.