Evidence map›Paper›PMID 28173868›Full record

ArticleLipids in health and disease2017

Docosahexaenoic acid increases accumulation of adipocyte triacylglycerol through up-regulation of lipogenic gene expression in pigs.

Chao-Wei Huang, Yu-Jen Chen, Jui-Ting Yang, Ching-Yi Chen, Kolapo M Ajuwon, Shuen-Ei Chen, Nan-Wei Su, Yu-Shan Chen, Harry J Mersmann, Shih-Torng Ding

Open access · goldAbstract read

PubMed Full text at PMC Publisher

In one paragraph

Article in Lipids in health and disease, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it

0cells of the map it votes in

8citing papers in PubMed

1.3field-weighted citation impact, top 24% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 14 citations in OpenAlex.

DHA ModulatesInternational journal of molecular sciences · 2025
Article
Docosahexaenoic Acid Suppresses Expression of Adipogenic Tetranectin through Sterol Regulatory Element-Binding Protein and Forkhead Box O Protein in Pigs.Nutrients · 2021
Article
Overexpression of Adiponectin Receptor 1 Inhibits Brown and Beige Adipose Tissue Activity in Mice.International journal of molecular sciences · 2021
Article
Short-Term Effects of Early Menopause on Adiposity, Fatty Acids Profile and Insulin Sensitivity of a Swine Model of Female Obesity.Biology · 2020
Article
Docosahexaenoic Acid (DHA) Induced Morphological Differentiation of Astrocytes Is Associated with Transcriptional Upregulation and Endocytosis of βMolecular neurobiology · 2019
Article
Effect of Eicosapentaenoic Acid and Docosahexaenoic Acid on Myogenesis and Mitochondrial Biosynthesis during Murine Skeletal Muscle Cell Differentiation.Frontiers in nutrition · 2018
Article
Effect of diets supplemented with linseed alone or combined with vitamin E and selenium or with plant extracts, onJournal of animal science and biotechnology · 2018
Article
Effect of dietary polyunsaturated fatty acid and antioxidant supplementation on the transcriptional level of genes involved in lipid and energy metabolism in swine.PloS one · 2018
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 2 countries.

Chao-Wei HuangDepartment of Animal Science and Technology, National Taiwan University, No.50, Ln. 155, Sec. 3, Keelung Rd., Da'an Dist, Taipei City, 10672, Taiwan.

Yu-Jen ChenInstitute of Biotechnology, National Taiwan University, Taipei, 10672, Taiwan.

Jui-Ting YangDepartment of Animal Science and Technology, National Taiwan University, No.50, Ln. 155, Sec. 3, Keelung Rd., Da'an Dist, Taipei City, 10672, Taiwan.

Ching-Yi ChenDepartment of Animal Science and Technology, National Taiwan University, No.50, Ln. 155, Sec. 3, Keelung Rd., Da'an Dist, Taipei City, 10672, Taiwan.

Kolapo M AjuwonDepartment of Animal Sciences, Purdue University, West Lafayette, IN, 47907-2054, USA.

Shuen-Ei ChenDepartment of Animal Science, National Chung-Hsing University, Taichung, 40227, Taiwan.

Nan-Wei SuDepartment of Agricultural Chemistry, National Taiwan University, Taipei, 10672, Taiwan.

Yu-Shan ChenDepartment of Animal Science and Technology, National Taiwan University, No.50, Ln. 155, Sec. 3, Keelung Rd., Da'an Dist, Taipei City, 10672, Taiwan.

Harry J MersmannDepartment of Animal Science and Technology, National Taiwan University, No.50, Ln. 155, Sec. 3, Keelung Rd., Da'an Dist, Taipei City, 10672, Taiwan.

Shih-Torng DingDepartment of Animal Science and Technology, National Taiwan University, No.50, Ln. 155, Sec. 3, Keelung Rd., Da'an Dist, Taipei City, 10672, Taiwan. sding@ntu.edu.tw.

National Taiwan University of Science and Technology · TWNational Taiwan University · TWNational Chung Hsing University · TWPurdue University West Lafayette · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundChanging dietary fatty acid composition in modern diet influences the prevalence of obesity. Increasing evidences suggest favorable effects of n-3 PUFA for protecting against obesity and the metabolic syndrome. However, the regulation of n-3 PUFA in adipose is still unclear. Thus, this study addressed metabolism of different dietary fats in the adipose tissue of porcine model.

methodsEight-week-old cross-bred pigs were randomly assigned to three groups and fed a 2% fat diet for 30 days from either soybean oil (SBO), docosahexaenoic acid (DHA) or beef tallow. An in vitro experiment was conducted in which linoleic acid (LA), DHA or oleic acid (OA) were added to represent the major fatty acid in the SBO-, DHA- or BT- diets, respectively. Adipocytes size and lipid metabolism related genes were analyzed.

resultsPlasma triacylglycerol (TAG) was lower in DHA- than in BT-fed pigs, and the product of lipolysis, glycerol was highest in BT-fed pigs. In addition, expression of the lipolytic genes, adipose triglyceride lipase and hormone sensitive lipase was higher in BT-fed pigs and with OA treatment in vitro. DHA promoted protein kinase A activity in pigs without affecting lipolytic genes. Adipocyte cell sizes, TAG content and expression of lipogenic-related genes including, adipose differentiated related protein (ADRP) and diacylglycerol acyltransferase 1 (DGAT1) were elevated by DHA in vivo and in vitro, indicating DHA promoted adipogenesis to trap TAG in adipose tissue. Fatty acid β-oxidation genes were increased in the DHA-fed pigs.

conclusionThis effect was partly explained by the effect of DHA to promote adipogenesis to trap TAG in adipocytes and also increase expression of genes involved in adipocyte fatty acid oxidation. Therefore, our results suggest a direct effect of DHA on adipocyte metabolism, resulting in TAG turnover and fatty acid dissipation to facilitate plasma lipid uptake from the circulation.

Indexed as

AdipocytesAdipogenesisAnimalsDietDocosahexaenoic AcidsFemaleLipogenesisMaleModels, AnimalObesityProteinsSwineTriglyceridesUp-RegulationDocosahexaenoic AcidsProteinsTriglyceridesAdipose tissueDocosahexaenoic acidLipid metabolismProtein kinase A

Identifiers

PMID28173868

DOI10.1186/s12944-017-0428-3

PMCPMC5297193

OpenAlexW2586094565

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.