ArticleBMC veterinary research2017
A novel multiplex qPCR targeting 23S rDNA for diagnosis of swine dysentery and porcine intestinal spirochaetosis.
Article in BMC veterinary research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 16 citations in OpenAlex.
- Brachyspira in dogs: risk factors of shedding in central Germany and longitudinal study of an infected kennel.BMC veterinary research · 2024Article
- Development of a TaqMan-based multiplex real-time PCR for simultaneous detection of porcine epidemic diarrhea virus,Frontiers in veterinary science · 2024Article
- Development and application of a quadruplexFrontiers in cellular and infection microbiology · 2024Article
- Novel multiplex TaqMan assay for differentiation of the four major pathogenic Brachyspira species in swine.MicrobiologyOpen · 2021Article
- The Spirochete Brachyspira pilosicoli, Enteric Pathogen of Animals and Humans.Clinical microbiology reviews · 2018Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA multiplex qPCR targeting a 128 bp region on the 23S rDNA gene was developed for detection of Brachyspira (B.) hyodysenteriae and B. pilosicoli, the agents of swine dysentery (SD) and porcine intestinal spirochaetosis (PIS), together with a triplet of apathogenic Brachyspira spp. (B. innocens, B. intermedia, B. murdochii) in porcine feces. The multiplex qPCR was evaluated against a duplex PCR (La et al., J Clin Microbiol 41:3372-5, 2003).
resultsUsing DNA extracted from fecal culture, the multiplex qPCR showed excellent agreement with the duplex PCR (κ = 0.943 and 0.933). In addition, thanks to the three probes whereof one detecting the apathogenic Brachyspria spp., a more diversified overview of the brachyspiral flora in porcine fecal samples can be delivered as a part of the routine diagnostic. The multiplex qPCR with a limit of detection of 5-10 genomic equivalents (GE) per reaction (6 × 10
conclusionThe novel multiplex qPCR is robust and has a high analytical sensitivity and is therefore suitable for high-throughput screening of porcine fecal swabs for the causative agents of SD. This assay can therefore be used for the direct proof of the pathogenic B. spp. in fecal swabs within the scope of a monitoring program.
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Registered trials
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