Evidence map›Paper›PMID 28173797›Full record

ArticleBMC cancer2017

Tumour-associated glial host cells display a stem-like phenotype with a distinct gene expression profile and promote growth of GBM xenografts.

Lina Leiss, Ercan Mutlu, Anne Øyan, Tao Yan, Oleg Tsinkalovsky, Linda Sleire, Kjell Petersen, Mohummad Aminur Rahman, Mireille Johannessen, Sidhartha S Mitra and 9 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC cancer, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 13 citations in OpenAlex.

  1. Crosstalk betweenInternational journal of molecular sciences · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 6 institutions in 4 countries.

Lina LeissNeuro Clinic, Haukeland University Hospital, Bergen, Norway.
Ercan MutluOncomatrix Research Lab, Department of Biomedicine, University of Bergen, Bergen, Norway. ercmut86@gmail.com.ORCID http://orcid.org/0000-0002-2513-4764
Anne ØyanDepartment of Clinical Science, University of Bergen, Bergen, Norway.
Tao YanOncomatrix Research Lab, Department of Biomedicine, University of Bergen, Bergen, Norway.
Oleg TsinkalovskyDepartment of Clinical Science, University of Bergen, Bergen, Norway.
Linda SleireOncomatrix Research Lab, Department of Biomedicine, University of Bergen, Bergen, Norway.
Kjell PetersenComputational Biology Unit, Uni Computing, Uni Research AS, Bergen, Norway.
Mohummad Aminur RahmanOncomatrix Research Lab, Department of Biomedicine, University of Bergen, Bergen, Norway.
Mireille JohannessenOncomatrix Research Lab, Department of Biomedicine, University of Bergen, Bergen, Norway.
Sidhartha S MitraInstitute for Stem Cell Biology and Regenerative Medicine, Stanford University, Stanford, California, USA.
Hege K JacobsenOncomatrix Research Lab, Department of Biomedicine, University of Bergen, Bergen, Norway.
Krishna M TalasilaTranslational Cancer Research Group, Department of Biomedicine, University of Bergen, Bergen, Norway.
Hrvoje MileticTranslational Cancer Research Group, Department of Biomedicine, University of Bergen, Bergen, Norway.
Inge JonassenComputational Biology Unit, Uni Computing, Uni Research AS, Bergen, Norway.
Xingang LiDepartment of Neurosurgery, Qilu Hospital of Shandong University, Jinan, China.
Nicolaas H BronsCore Facility Flow Cytometry, Centre de Recherche Public de la Santé (CRP-Santé), L-1526, Luxembourg, Luxembourg.
Karl-Henning KallandDepartment of Clinical Science, University of Bergen, Bergen, Norway.
Jian WangOncomatrix Research Lab, Department of Biomedicine, University of Bergen, Bergen, Norway.
Per Øyvind EngerOncomatrix Research Lab, Department of Biomedicine, University of Bergen, Bergen, Norway.
University of Bergen · NOHaukeland University Hospital · NOShandong University · CNUni Research (Norway) · NOCalifornia Institute for Regenerative Medicine · USLaboratoire National de Santé · LU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundLittle is known about the role of glial host cells in brain tumours. However, supporting stromal cells have been shown to foster tumour growth in other cancers.

methodsWe isolated stromal cells from patient-derived glioblastoma (GBM) xenografts established in GFP-NOD/scid mice. With simultaneous removal of CD11b

resultsTAGs were highly enriched for the expression of glial cell proteins including GFAP and myelin basic protein (MBP), and immature markers such as Nestin and O4. A fraction of TAGs displayed sphere formation in stem cell medium. Moreover, TAGs promoted brain tumour growth in vivo when co-implanted with glioma cells, compared to implanting only glioma cells, or glioma cells and unconditioned glial cells from mice without tumours. Genome-wide microarray analysis of TAGs showed an expression profile distinct from glial cells from healthy mice brains. Notably, TAGs upregulated genes associated with immature cell types and self-renewal, including Pou3f2 and Sox2. In addition, TAGs from highly angiogenic tumours showed upregulation of angiogenic factors, including Vegf and Angiopoietin 2. Immunohistochemistry of three GBMs, two patient biopsies and one GBM xenograft, confirmed that the expression of these genes was mainly confined to TAGs in the tumour bed. Furthermore, their expression profiles displayed a significant overlap with gene clusters defining prognostic subclasses of human GBMs.

conclusionsOur data demonstrate that glial host cells in brain tumours are functionally distinct from glial cells of healthy mice brains. Furthermore, TAGs display a gene expression profile with enrichment for genes related to stem cells, immature cell types and developmental processes. Future studies are needed to delineate the biological mechanisms regulating the brain tumour-host interplay.

Indexed as

TranscriptomeAnimalsBiomarkers, TumorBrainBrain NeoplasmsCell Line, TumorGene Expression Regulation, NeoplasticGlioblastomaHumansImmunohistochemistryMiceMice, Inbred NODMice, SCIDMicroarray AnalysisXenograft Model Antitumor AssaysBiomarkers, TumorGene expression analysisGFF-NOD/scid miceGlioblastomaPOU3F2Stem cell markersTumour-associated glial cellsTumour-host interplayXenograft tumours

Identifiers

PMID28173797
PMCPMC5294893
OpenAlexW2587633146

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.