Evidence map›Paper›PMID 28173622›Full record

ArticleFEBS letters2017

Osteoclast precursors do not express CD68: results from CD68 promoter-driven RANK transgenic mice.

Melissa F Jackson, Marta Scatena, Cecilia M Giachelli

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS letters, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.4field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 15 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Melissa F JacksonDepartment of Bioengineering, University of Washington, Seattle, WA, USA.
Marta ScatenaDepartment of Bioengineering, University of Washington, Seattle, WA, USA.
Cecilia M GiachelliDepartment of Bioengineering, University of Washington, Seattle, WA, USA.
University of Washington · US

Funding

Vector and Transgenic Mouse CoreP30DK017047 · NIDDK · UNIVERSITY OF WASHINGTON · PI Sakeneh Zraika · 1986 to 2026
$41.4M
Calcification and Vascular Cell DifferentiationR01HL081785 · NHLBI · UNIVERSITY OF WASHINGTON · PI GIACHELLI, CECILIA M · 2005 to 2017
$3.7M
Role of Osteoclastogenesis in Calcific Aortic Valve DiseaseR01HL114611 · NHLBI · UNIVERSITY OF WASHINGTON · PI GIACHELLI, CECILIA M · 2012 to 2016
$1.9M
NHLBI NIH HHS R01 HL081785NHLBI NIH HHS R01 HL114611NIDDK NIH HHS P30 DK017047
6 · The paper itself

Abstract

Macrophages and osteoclasts are thought to derive from CD68 lineage marker-positive common myeloid precursors. We used the CD68 promoter to drive an inducible receptor activator of NF-κB (iRANK) construct that selectively activates RANK signaling in myeloid cells in vivo. The cytoplasmic portion of RANK was fused to a mutant FK506 binding domain, which selectively binds the chemical inducer of dimerization AP20187 and initiates signaling. iRANK mRNA was expressed in macrophages isolated from peritoneal cavity, spleen-, and bone marrow-derived myeloid cells. Unexpectedly, AP20187 did not induce osteoclast formation in spleen- and bone marrow-derived myeloid cells. However, AP20187-dependent RANK signaling induced ERK1/2 phosphorylation and mRNA expression of MMP9 and CathepsinK in peritoneal macrophages. Importantly, CD68 was not expressed until day 3 and day 5 in bone marrow and spleen myeloid cells, respectively. Contrary to dogma, osteoclast precursors do not express the lineage marker CD68.

Indexed as

AnimalsAntigens, CDAntigens, Differentiation, MyelomonocyticBone Marrow CellsCathepsin KCD68 MoleculeCell DifferentiationGene Expression RegulationMacrophagesMatrix Metalloproteinase 9MiceMice, TransgenicMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Myeloid CellsOsteoclastsAntigens, CDAntigens, Differentiation, MyelomonocyticAP20187Cathepsin KCD68 MoleculeCD68 protein, mouseCtsk protein, mouseMapk1 protein, mouseMatrix Metalloproteinase 9Mitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mmp9 protein, mouseReceptor Activator of Nuclear Factor-kappa BRecombinant Fusion ProteinsTacrolimusTacrolimus Binding ProteinsTnfrsf11a protein, mouseCD68osteoclast precursorsRANK

Identifiers

PMID28173622
PMCPMC5391845
OpenAlexW2586892090

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.