Evidence map›Paper›PMID 28170537›Full record

ArticleInvestigative ophthalmology & visual science2017

Adenosine Deaminase-2-Induced Hyperpermeability in Human Retinal Vascular Endothelial Cells Is Suppressed by MicroRNA-146b-3p.

Yara A Samra, Heba M Saleh, Khaled A Hussein, Nehal M Elsherbiny, Ahmed S Ibrahim, Khaled Elmasry, Sadanand Fulzele, Mamdouh M El-Shishtawy, Laila A Eissa, Mohamed Al-Shabrawey and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Investigative ophthalmology & visual science, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
2.8field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 27 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 3 countries.

Yara A SamraDepartment of Biochemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt 2Department of Ophthalmology and The Culver Vision Discovery Institute, Medical College of Georgia, Augusta University, Augusta, Georgia, United States.
Heba M SalehDepartment of Oral Biology/Anatomy, Dental College of Georgia, Augusta University, Augusta, Georgia, United States.
Khaled A HusseinDepartment of Oral Biology/Anatomy, Dental College of Georgia, Augusta University, Augusta, Georgia, United States 4Department of Oral Medicine and Surgery, Oral and Dental Research Division, National Research Centre, Cairo, Egypt.
Nehal M ElsherbinyDepartment of Biochemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt 3Department of Oral Biology/Anatomy, Dental College of Georgia, Augusta University, Augusta, Georgia, United States 5Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Tabuk, Tabuk, Saudi Arabia.
Ahmed S IbrahimDepartment of Biochemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt 3Department of Oral Biology/Anatomy, Dental College of Georgia, Augusta University, Augusta, Georgia, United States.
Khaled ElmasryDepartment of Oral Biology/Anatomy, Dental College of Georgia, Augusta University, Augusta, Georgia, United States 6Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, Augusta, Georgia, United States 7Department of Anatomy, Faculty of Medicine, Mansoura University, Egypt.
Sadanand FulzeleDepartment of Orthopedics, Medical College of Georgia, Augusta University, Augusta, Georgia, United States.
Mamdouh M El-ShishtawyDepartment of Biochemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
Laila A EissaDepartment of Biochemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
Mohamed Al-ShabraweyDepartment of Ophthalmology and The Culver Vision Discovery Institute, Medical College of Georgia, Augusta University, Augusta, Georgia, United States 3Department of Oral Biology/Anatomy, Dental College of Georgia, Augusta University, Augusta, Georgia, United States 6Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, Augusta, Georgia, United States 7Department of Anatomy, Faculty of Medicine, Mansoura University, Egypt.
Gregory I LiouDepartment of Ophthalmology and The Culver Vision Discovery Institute, Medical College of Georgia, Augusta University, Augusta, Georgia, United States.
Mansoura University · EGAugusta University · USDiscovery Institute · USNational Research Centre · EGUniversity of Tabuk · SA

Funding

Role of lipoxygenase pathway in early microvascular dysfunction during diabetic rR01EY023315 · NEI · AUGUSTA UNIVERSITY · PI AL-SHABRAWEY, MOHAMED AL-SAYED · 2013 to 2017
$1.8M
NEI NIH HHS R01 EY023315
6 · The paper itself

Abstract

Purpose: We recently demonstrated that adenosine deaminase-2 (ADA2) contributes to diabetic retinopathy (DR) via up-regulating the production of inflammatory cytokines in macrophages. Also, microRNA (miR)-146b-3p has the ability to inhibit ADA2. The goal of this study was to investigate the potential role of ADA2 and therapeutic benefit of miR-146b-3p in retinal inflammation and endothelial barrier dysfunction during diabetes. Methods: Adenosine deaminase-2 activity was determined by colorimetric method in diabetic human vitreous. Human monocyte cell line U937 was differentiated into macrophages and then treated with amadori glycated albumin (AGA), and conditioned medium (CM) was used to assess the changes in ADA2 activity and TNF-α and IL-6 levels by ELISA. Also, macrophages were transfected with miR-146b-3p before treatment with AGA. Permeability of human retinal endothelial cells (hRECs) was assessed by electric cell-substrate impedance sensing (ECIS) after treatment with macrophage CM. Zonula occludens (ZO)-1 was examined by immuno-fluorescence in hRECs. Leukocyte adhesion was assessed in hRECs by measuring myeloperoxidase (MPO) activity and intercellular adhesion molecule-1 (ICAM-1) expression. Results: Adenosine deaminase-2 activity was significantly increased in diabetic human vitreous. ADA2 activity and TNF-α and IL-6 levels were significantly increased in human macrophages by AGA treatment. Amadori glycated albumin-treated macrophage CM significantly increased hREC permeability, disrupted ZO-1 pattern, and increased leukocyte adhesion to hRECs through up-regulating ICAM-1. All these changes were reversed by miR-146b-3p. Conclusions: Adenosine deaminase-2 is implicated in breakdown of the blood-retinal barrier (BRB) in DR through macrophages-derived cytokines. Therefore, inhibition of ADA2 by miR-146b-3p might be a useful tool to preserve BRB function in DR.

Indexed as

Adenosine DeaminaseBlotting, WesternCapillary PermeabilityCell LineCytokinesDiabetic RetinopathyEndothelial CellsEnzyme-Linked Immunosorbent AssayGlycated Serum AlbuminGlycation End Products, AdvancedHumansMicroRNAsRetinaRetinal VesselsSerum AlbuminTight JunctionsAdenosine DeaminaseCytokinesGlycated Serum AlbuminGlycation End Products, AdvancedMicroRNAsMIRN146 microRNA, humanSerum Albumin

Identifiers

PMID28170537
PMCPMC5300249
OpenAlexW2586723687

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.