ArticleThe journal of physical chemistry. B2017
Wavelength-Dependent Second Harmonic Generation Circular Dichroism for Differentiation of Col I and Col III Isoforms in Stromal Models of Ovarian Cancer Based on Intrinsic Chirality Differences.
Article in The journal of physical chemistry. B, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 40 citations in OpenAlex.
- Generalized 3D quasi-phase-matching model of image contrast in second harmonic generation microscopy of fibrillar collagen architectures.JPhys photonics · 2026Article
- Synergistic Effects of Non-Ionizing Radiation in the Targeted Modification of Living Tissues.International journal of molecular sciences · 2025Review
- A Perspective Review: Analyzing Collagen Alterations in Ovarian Cancer by High-Resolution Optical Microscopy.Cancers · 2024Review
- Effect of out of plane orientation on polarization second harmonic generation of single collagen fibrils.Biomedical optics express · 2023Article
- Unsupervised determination of lung tumor margin with widefield polarimetric second-harmonic generation microscopy.Scientific reports · 2022Article
- Characterization of collagen response to bone fracture healing using polarization-SHG.Scientific reports · 2022Article
- Machine learning-enabled cancer diagnostics with widefield polarimetric second-harmonic generation microscopy.Scientific reports · 2022Article
- Assessing collagen alterations in enzymatic degradation models of osteoarthritis via second harmonic generation microscopy.Osteoarthritis and cartilage · 2021Article
- Examining lysyl oxidase-like modulation of collagen architecture in 3D spheroid models of idiopathic pulmonary fibrosis via second-harmonic generation microscopy.Journal of biomedical optics · 2021Article
- Dual-LC PSHG microscopy for imaging collagen type I and type II gels with pixel-resolution analysis.Biomedical optics express · 2021Article
- Recent Advancements in Optical Harmonic Generation Microscopy: Applications and Perspectives.BME frontiers · 2021Review
- Probing ECM remodeling in idiopathic pulmonary fibrosis via second harmonic generation microscopy analysis of macro/supramolecular collagen structure.Journal of biomedical optics · 2019Article
- Textures of the tumour microenvironment.Essays in biochemistry · 2019Review
- Complex Susceptibilities and Chiroptical Effects of Collagen Measured with Polarimetric Second-Harmonic Generation Microscopy.Scientific reports · 2019Article
- Exploring Collagen Parameters in Pure Special Types of Invasive Breast Cancer.Scientific reports · 2019Article
- Characterization of Pancreatic Cancer Tissue Using Multiphoton Excitation Fluorescence and Polarization-Sensitive Harmonic Generation Microscopy.Frontiers in oncology · 2019Article
- Polarization-resolved second harmonic generation imaging of human ovarian cancer.Journal of biomedical optics · 2018Article
- Assessing local stromal alterations in human ovarian cancer subtypes via second harmonic generation microscopy and analysis.Journal of biomedical optics · 2017Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Extensive remodeling of the extracellular matrix (ECM) occurs in many epithelial cancers. For example, in ovarian cancer, upregulation of collagen isoform type III has been linked to invasive forms of the disease, and this change may be a potential biomarker. To examine this possibility, we implemented wavelength-dependent second harmonic generation circular dichroism (SHG-CD) imaging microscopy to quantitatively determine changes in chirality in ECM models comprised of different Col I/Col III composition. In these models, Col III was varied between 0 and 40%, and we found increasing Col III results in reduced net chirality, consistent with structural biology studies of Col I and III in tissues where the isoforms comingle in the same fibrils. We further examined the wavelength dependence of the SHG-CD to both optimize the response and gain insight into the underlying mechanism. We found using shorter SHG excitation wavelengths resulted in increased SHG-CD sensitivity, where this is consistent with the electric-dipole-coupled oscillator model suggested previously for the nonlinear chirality response from thin films. Moreover, the sensitivity is further consistent with the wavelength dependency of SHG intensity fit to a two-state model of the two-photon absorption in collagen. We also provide experimental calibration protocols to implement the SHG-CD modality on a laser scanning microscope. We last suggest that the technique has broad applicability in probing a wide range of diseased states with changes in collagen molecular structure.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.