ArticleeLife2017
Design principles of autocatalytic cycles constrain enzyme kinetics and force low substrate saturation at flux branch points.
Article in eLife, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 92 citations in OpenAlex.
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- Using MR-chordless circuits for efficient enumeration of autocatalytic cores in large chemical reaction networks.Journal of cheminformatics · 2026Article
- Enumeration of Autocatalytic Subsystems in Large Chemical Reaction Networks.Journal of chemical theory and computation · 2026Article
- Computational approaches in chemical space exploration for carbon fixation pathways.NPJ systems biology and applications · 2026Article
- Multicriteria evaluation of ethylene glycol assimilation pathways.Journal of industrial microbiology & biotechnology · 2026Article
- The Nature of Organization in Living Systems.ArXiv · 2025Article
- Seven critical challenges in synthetic one-carbon assimilation and their potential solutions.FEMS microbiology reviews · 2025Review
- Free-energy transduction mechanisms shape the flux space of metabolic networks.Biophysical journal · 2024Article
- Phosphoribosylpyrophosphate synthetase as a metabolic valve advances Methylobacterium/Methylorubrum phyllosphere colonization and plant growth.Nature communications · 2024Article
- Constructing efficient bacterial cell factories to enable one-carbon utilization based on quantitative biology: A review.Quantitative biology (Beijing, China) · 2024Review
- Autotrophic growth ofeLife · 2024Article
- Synergistic investigation of natural and synthetic C1-trophic microorganisms to foster a circular carbon economy.Nature communications · 2023Review
- Metabolite interactions in the bacterial Calvin cycle and implications for flux regulation.Communications biology · 2023Article
- Article
- Characterization of the enzyme kinetics of EMP and HMP pathway inFrontiers in bioengineering and biotechnology · 2023Article
- Design and Construction of Artificial Biological Systems for One-Carbon Utilization.Biodesign research · 2023Review
- Growth and depletion in linear stochastic reaction networks.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Generation of an Escherichia coli strain growing on methanol via the ribulose monophosphate cycle.Nature communications · 2022Article
- Two major metabolic factors for an efficient NADP-malic enzyme type C4 photosynthesis.Plant physiology · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A set of chemical reactions that require a metabolite to synthesize more of that metabolite is an autocatalytic cycle. Here, we show that most of the reactions in the core of central carbon metabolism are part of compact autocatalytic cycles. Such metabolic designs must meet specific conditions to support stable fluxes, hence avoiding depletion of intermediate metabolites. As such, they are subjected to constraints that may seem counter-intuitive: the enzymes of branch reactions out of the cycle must be overexpressed and the affinity of these enzymes to their substrates must be relatively weak. We use recent quantitative proteomics and fluxomics measurements to show that the above conditions hold for functioning cycles in central carbon metabolism of
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.