ArticleScientific reports2017
Enhanced delignification of steam-pretreated poplar by a bacterial laccase.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 5 citations in OpenAlex.
- Sustainable Pretreatment of Lignocellulosic Biomass for Biohydrogen Production.Molecules (Basel, Switzerland) · 2026Review
- Laccase-catalyzed conversion of residual agricultural biomass to lignin-derived aromatic compounds.World journal of microbiology & biotechnology · 2025Article
- Enzymatic Treatment of Lignin in Alkaline Homogeneous Systems: A Review on Alkaliphilic Laccases.ChemSusChem · 2025Review
- Discovery of lignin-transforming bacteria and enzymes in thermophilic environments using stable isotope probing.The ISME journal · 2022Article
- Article
- Isolation and Characterization of a Novel Laccase for Lignin Degradation, LacZ1.Applied and environmental microbiology · 2021Article
- Laccase-mediated delignification and detoxification of lignocellulosic biomass: removing obstacles in energy generation.Environmental science and pollution research international · 2021Review
- Comparison of performances of different fungal laccases in delignification and detoxification of alkali-pretreated corncob for bioethanol production.Journal of industrial microbiology & biotechnology · 2021Article
- Metabolic engineering of Rhodococcus jostii RHA1 for production of pyridine-dicarboxylic acids from lignin.Microbial cell factories · 2021Article
- The Comparative Abilities of a Small Laccase and a Dye-Decoloring Peroxidase From the Same Bacterium to Transform Natural and Technical Lignins.Frontiers in microbiology · 2021Article
- A first report on competitive inhibition of laccase enzyme by lignin degradation intermediates.Folia microbiologica · 2020Article
- Article
- Oxidoreductases and Reactive Oxygen Species in Conversion of Lignocellulosic Biomass.Microbiology and molecular biology reviews : MMBR · 2018Review
- Bacterial laccases: promising biological green tools for industrial applications.Cellular and molecular life sciences : CMLS · 2018Review
- Getting Closer to Absolute Molar Masses of Technical Lignins.ChemSusChem · 2018Article
- Pre-treatment of Oil Palm Biomass for Fermentable Sugars Production.Molecules (Basel, Switzerland) · 2018Review
- Multiple Reaction Monitoring for quantitative laccase kinetics by LC-MS.Scientific reports · 2018Article
- Evaluation of Screened Lignin-degrading Fungi for the Biological Pretreatment of Corn Stover.Scientific reports · 2018Article
- Laccase-derived lignin compounds boost cellulose oxidative enzymes AA9.Biotechnology for biofuels · 2018Article
- Comparison of the efficiency of bacterial and fungal laccases in delignification and detoxification of steam-pretreated lignocellulosic biomass for bioethanol production.Journal of industrial microbiology & biotechnology · 2017Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The recalcitrance of woody biomass, particularly its lignin component, hinders its sustainable transformation to fuels and biomaterials. Although the recent discovery of several bacterial ligninases promises the development of novel biocatalysts, these enzymes have largely been characterized using model substrates: direct evidence for their action on biomass is lacking. Herein, we report the delignification of woody biomass by a small laccase (sLac) from Amycolatopsis sp. 75iv3. Incubation of steam-pretreated poplar (SPP) with sLac enhanced the release of acid-precipitable polymeric lignin (APPL) by ~6-fold, and reduced the amount of acid-soluble lignin by ~15%. NMR spectrometry revealed that the APPL was significantly syringyl-enriched relative to the original material (~16:1 vs. ~3:1), and that sLac preferentially oxidized syringyl units and altered interunit linkage distributions. sLac's substrate preference among monoaryls was also consistent with this observation. In addition, sLac treatment reduced the molar mass of the APPL by over 50%, as determined by gel-permeation chromatography coupled with multi-angle light scattering. Finally, sLac acted synergistically with a commercial cellulase cocktail to increase glucose production from SPP ~8%. Overall, this study establishes the lignolytic activity of sLac on woody biomass and highlights the biocatalytic potential of bacterial enzymes.
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