ArticleScientific reports2017
Correlative Imaging of the Murine Hind Limb Vasculature and Muscle Tissue by MicroCT and Light Microscopy.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Predicting cortical bone resorption in the mouse tibia under disuse conditions caused by transient muscle paralysis.Scientific reports · 2025Article
- In situ isotropic 3D imaging of vasculature perfusion specimens using x-ray microscopic dual-energy CT.Journal of microscopy · 2025Article
- Contrast-Enhanced Ultrasound of Mouse Models of Hindlimb Ischemia Reveals Persistent Perfusion Deficits and Distinctive Muscle Perfusion Patterns.Ultrasound in medicine & biology · 2025Article
- Multiscale Three-Dimensional Evaluation and Analysis of Murine Lung Vasculature From Macro- to Micro-Structural Level.Pulmonary circulation · 2025Article
- High resolution imaging of human development: shedding light on contrast agents.Neuroradiology · 2024Review
- Skeletal muscle myosin heavy chain expression and 3D capillary network changes in streptozotocin-induced diabetic female mice.Biomolecules & biomedicine · 2024Article
- Comparison of micro-CT image enhancement after use of different vascular casting agents.Quantitative imaging in medicine and surgery · 2024Article
- High-resolution micro-CT for 3D infarct characterization and segmentation in mice stroke models.Scientific reports · 2022Article
- Open-source analysis and visualization of segmented vasculature datasets with VesselVio.Cell reports methods · 2022Article
- Intravoxel Incoherent Motion Magnetic Resonance Imaging in Skeletal Muscle: Review and Future Directions.Journal of magnetic resonance imaging : JMRI · 2022Review
- VascuViz: a multimodality and multiscale imaging and visualization pipeline for vascular systems biology.Nature methods · 2022Article
- Skeletal Muscle Microvascular Dysfunction in Obesity-Related Insulin Resistance: Pathophysiological Mechanisms and Therapeutic Perspectives.International journal of molecular sciences · 2022Review
- Post-mortem Computed Tomographic Angiography in Equine Distal Forelimbs: A Feasibility Study.Frontiers in veterinary science · 2022Article
- Evaluation of 2D super-resolution ultrasound imaging of the rat renal vasculature using ex vivo micro-computed tomography.Scientific reports · 2021Article
- Not All Lectins Are Equally Suitable for Labeling Rodent Vasculature.International journal of molecular sciences · 2021Article
- Micro-CT for Biological and Biomedical Studies: A Comparison of Imaging Techniques.Journal of imaging · 2021Review
- Parsimonious modeling of skeletal muscle perfusion: Connecting the stretched exponential and fractional Fickian diffusion.Magnetic resonance in medicine · 2021Article
- The C5a/C5a receptor 1 axis controls tissue neovascularization through CXCL4 release from platelets.Nature communications · 2021Article
- Review
- A Review of Ex Vivo X-ray Microfocus Computed Tomography-Based Characterization of the Cardiovascular System.International journal of molecular sciences · 2021Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A detailed vascular visualization and adequate quantification is essential for the proper assessment of novel angiomodulating strategies. Here, we introduce an ex vivo micro-computed tomography (microCT)-based imaging approach for the 3D visualization of the entire vasculature down to the capillary level and rapid estimation of the vascular volume and vessel size distribution. After perfusion with μAngiofil®, a novel polymerizing contrast agent, low- and high-resolution scans (voxel side length: 2.58-0.66 μm) of the entire vasculature were acquired. Based on the microCT data, sites of interest were defined and samples further processed for correlative morphology. The solidified, autofluorescent μAngiofil® remained in the vasculature and allowed co-registering of the histological sections with the corresponding microCT-stack. The perfusion efficiency of μAngiofil® was validated based on lectin-stained histological sections: 98 ± 0.5% of the blood vessels were μAngiofil®-positive, whereas 93 ± 2.6% were lectin-positive. By applying this approach we analyzed the angiogenesis induced by the cell-based delivery of a controlled VEGF dose. Vascular density increased by 426% mainly through the augmentation of medium-sized vessels (20-40 μm). The introduced correlative and quantitative imaging approach is highly reproducible and allows a detailed 3D characterization of the vasculature and muscle tissue. Combined with histology, a broad range of complementary structural information can be obtained.
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