ArticleAMB Express2017
Increasing cocoa butter-like lipid production of Saccharomyces cerevisiae by expression of selected cocoa genes.
Article in AMB Express, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 29 citations in OpenAlex.
- Sustainable industrial crop-based strategies for cocoa butter substitutes: advances in plant, microbial, and lipid engineering.Food chemistry: X · 2026Review
- Article
- Advances in synthesizing plant-derived isoflavones and their precursors with multiple pharmacological activities using engineered yeasts.Microbial cell factories · 2025Review
- Metabolic Engineering Strategies for Improved Lipid Production and Cellular Physiological Responses in YeastJournal of fungi (Basel, Switzerland) · 2022Review
- Altering the fatty acid profile of Yarrowia lipolytica to mimic cocoa butter by genetic engineering of desaturases.Microbial cell factories · 2022Article
- Yeast Synthetic Biology for the Production ofMolecules (Basel, Switzerland) · 2021Review
- Developments in Fatty Acid-Derived Insect Pheromone Production Using Engineered Yeasts.Frontiers in microbiology · 2021Review
- Editorial: Engineering Yeast to Produce Plant Natural Products.Frontiers in bioengineering and biotechnology · 2021Article
- Metabolic Engineering for Glycyrrhetinic Acid Production inFrontiers in bioengineering and biotechnology · 2020Review
- Advances in Metabolic Engineering ofFrontiers in bioengineering and biotechnology · 2020Review
- Quantitative Mapping of Triacylglycerol Chain Length and Saturation Using Broadband CARS Microscopy.Biophysical journal · 2019Article
- Identification of genes involved in shea butter biosynthesis from Vitellaria paradoxa fruits through transcriptomics and functional heterologous expression.Applied microbiology and biotechnology · 2019Article
- Metabolic engineering ofMetabolic engineering communications · 2018Article
- Expression of cocoa genes in Saccharomyces cerevisiae improves cocoa butter production.Microbial cell factories · 2018Article
- Systems and Synthetic Biology Approaches to Engineer Fungi for Fine Chemical Production.Frontiers in bioengineering and biotechnology · 2018Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cocoa butter (CB) extracted from cocoa beans mainly consists of three different kinds of triacylglycerols (TAGs), 1,3-dipalmitoyl-2-oleoyl-glycerol (POP, C16:0-C18:1-C16:0), 1-palmitoyl-3-stearoyl-2-oleoyl-glycerol (POS, C16:0-C18:1-C18:0) and 1,3-distearoyl-2-oleoyl-glycerol (SOS, C18:0-C18:1-C18:0), but CB supply is limited. Therefore, CB-like lipids (CBL, which are composed of POP, POS and SOS) are in great demand. Saccharomyces cerevisiae produces TAGs as storage lipids, which are also mainly composed of C16 and C18 fatty acids. However, POP, POS and SOS are not among the major TAG forms in yeast. TAG synthesis is mainly catalyzed by three enzymes: glycerol-3-phosphate acyltransferase (GPAT), lysophospholipid acyltransferase (LPAT) and diacylglycerol acyltransferase (DGAT). In order to produce CBL in S. cerevisiae, we selected six cocoa genes encoding GPAT, LPAT and DGAT potentially responsible for CB biosynthesis from the cocoa genome using a phylogenetic analysis approach. By expressing the selected cocoa genes in S. cerevisiae, we successfully increased total fatty acid production, TAG production and CBL production in some S. cerevisiae strains. The relative CBL content in three yeast strains harboring cocoa genes increased 190, 230 and 196% over the control strain, respectively; especially, the potential SOS content of the three yeast strains increased 254, 476 and 354% over the control strain. Moreover, one of the three yeast strains had a 2.25-fold increased TAG content and 6.7-fold higher level of CBL compared with the control strain. In summary, CBL production by S. cerevisiae were increased through expressing selected cocoa genes potentially involved in CB biosynthesis.
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