ArticleScientific reports2017
SpotLight Proteomics: uncovering the hidden blood proteome improves diagnostic power of proteomics.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 29 citations in OpenAlex.
- Translational bioinformatics and data science for biomarker discovery in mental health: an analytical review.Briefings in bioinformatics · 2024Review
- Review
- Template-Based Assembly of Proteomic Short Reads ForAnalytical chemistry · 2022Review
- Proteomic Characterization of the Olfactory Molecular Imbalance in Dementia with Lewy Bodies.International journal of molecular sciences · 2020Article
- IgM antibodies against malondialdehyde and phosphorylcholine in different systemic rheumatic diseases.Scientific reports · 2020Article
- Focus on the spectra that matter by clustering of quantification data in shotgun proteomics.Nature communications · 2020Article
- Immunoglobulin G1 Antibodies Against Phosphorylcholine Are Associated With Protection in Systemic Lupus Erythematosus and Atherosclerosis: Potential Underlying Mechanisms.ACR open rheumatology · 2020Article
- The Proteomic Landscape of Pancreatic Ductal Adenocarcinoma Liver Metastases Identifies Molecular Subtypes and Associations with Clinical Response.Clinical cancer research : an official journal of the American Association for Cancer Research · 2020Article
- SpotLight Proteomics-A IgG-Enrichment Phenotype Profiling Approach with Clinical Implications.International journal of molecular sciences · 2019Article
- Amyloidogenic Nanoplaques in Blood Serum of Patients with Alzheimer's Disease Revealed by Time-Resolved Thioflavin T Fluorescence Intensity Fluctuation Analysis.Journal of Alzheimer's disease : JAD · 2019Article
- Altered Fc galactosylation in IgGERJ open research · 2018Article
- Covariation of Peptide Abundances Accurately Reflects Protein Concentration Differences.Molecular & cellular proteomics : MCP · 2017Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human blood proteome is frequently assessed by protein abundance profiling using a combination of liquid chromatography and tandem mass spectrometry (LC-MS/MS). In traditional sequence database search, many good-quality MS/MS data remain unassigned. Here we uncover the hidden part of the blood proteome via novel SpotLight approach. This method combines de novo MS/MS sequencing of enriched antibodies and co-extracted proteins with subsequent label-free quantification of new and known peptides in both enriched and unfractionated samples. In a pilot study on differentiating early stages of Alzheimer's disease (AD) from Dementia with Lewy Bodies (DLB), on peptide level the hidden proteome contributed almost as much information to patient stratification as the apparent proteome. Intriguingly, many of the new peptide sequences are attributable to antibody variable regions, and are potentially indicative of disease etiology. When the hidden and apparent proteomes are combined, the accuracy of differentiating AD (n = 97) and DLB (n = 47) increased from ≈85% to ≈95%. The low added burden of SpotLight proteome analysis makes it attractive for use in clinical settings.
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