ArticleProceedings of the National Academy of Sciences of the United States of America2017
iPSCs and fibroblast subclones from the same fibroblast population contain comparable levels of sequence variations.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Advances in Induced Pluripotent Stem Cell Reprogramming and Its Application in Amyotrophic Lateral Sclerosis: A Review.FASEB bioAdvances · 2025Review
- Transcriptome and epigenome dynamics of the clonal heterogeneity of human induced pluripotent stem cells for cardiac differentiation.Cellular and molecular life sciences : CMLS · 2024Article
- Genetic confounds of transgenerational epigenetic inheritance in mice.Epigenetics · 2024Article
- iPS cell generation-associated point mutations include many C > T substitutions via different cytosine modification mechanisms.Nature communications · 2024Article
- Article
- Replication stress causes delayed mitotic entry and chromosome 12 fragility at the ANKS1B large neuronal gene in human induced pluripotent stem cells.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2023Article
- Clonally Selected Lines After CRISPR-Cas Editing Are Not Isogenic.The CRISPR journal · 2023Article
- Dental applications of induced pluripotent stem cells and their derivatives.The Japanese dental science review · 2022Review
- Integrative network analysis of circular RNAs reveals regulatory mechanisms for hepatic specification of human iPSC-derived endoderm.Stem cell research & therapy · 2022Article
- Substantial somatic genomic variation and selection for BCOR mutations in human induced pluripotent stem cells.Nature genetics · 2022Article
- Human Induced Pluripotent Stem Cells: From Cell Origin, Genomic Stability, and Epigenetic Memory to Translational Medicine.Stem cells (Dayton, Ohio) · 2022Review
- Cellular and Engineered Organoids for Cardiovascular Models.Circulation research · 2022Review
- 'Channeling' therapeutic discovery for epileptic encephalopathy through iPSC technologies.Trends in pharmacological sciences · 2022Review
- SomaMutDB: a database of somatic mutations in normal human tissues.Nucleic acids research · 2022Article
- Ocular stem cells: a narrative review of current clinical trials.International journal of ophthalmology · 2022Review
- Patient-derived iPSCs link elevated mitochondrial respiratory complex I function to osteosarcoma in Rothmund-Thomson syndrome.PLoS genetics · 2021Article
- Reprogramming enriches for somatic cell clones with small-scale mutations in cancer-associated genes.Molecular therapy : the journal of the American Society of Gene Therapy · 2021Article
- Complex biology of constitutional ring chromosomes structure and (in)stability revealed by somatic cell reprogramming.Scientific reports · 2021Article
- Stem cell transplantation for treating liver diseases: progress and remaining challenges.American journal of translational research · 2021Review
- The mutational impact of culturing human pluripotent and adult stem cells.Nature communications · 2020Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Genome integrity of induced pluripotent stem cells (iPSCs) has been extensively studied in recent years, but it is still unclear whether iPSCs contain more genomic variations than cultured somatic cells. One important question is the origin of genomic variations detected in iPSCs-whether iPSC reprogramming induces such variations. Here, we undertook a unique approach by deriving fibroblast subclones and clonal iPSC lines from the same fibroblast population and applied next-generation sequencing to compare genomic variations in these lines. Targeted deep sequencing of parental fibroblasts revealed that most variants detected in clonal iPSCs and fibroblast subclones were rare variants inherited from the parental fibroblasts. Only a small number of variants remained undetectable in the parental fibroblasts, which were thus likely to be de novo. Importantly, the clonal iPSCs and fibroblast subclones contained comparable numbers of de novo variants. Collectively, our data suggest that iPSC reprogramming is not mutagenic.
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