ArticleBMC genomics2017
Analysis of small RNAs revealed differential expressions during pollen and embryo sac development in autotetraploid rice.
Article in BMC genomics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 80 citations in OpenAlex.
- Comparative physiological, hormonal, and transcriptional analyses reveal the difference between diploid and autotetraploid rice under cadmium stress.Plant cell reports · 2026Article
- HSP101-encoding NEO-TETRAPLOID RICE FERTILITY GENE 1 regulates tapetum development through interaction with SAPK2 in polyploid rice.Journal of integrative plant biology · 2026Article
- Exploring the Diversity of Ovule Development in the Novel Rice MutantPlants (Basel, Switzerland) · 2025Article
- Identification of a Novel Rice Chromosomal Translocation Line that Could Cause the Heterozygote Semi-Sterility and be Overcome by Genomic Duplication.Rice (New York, N.Y.) · 2025Article
- Comparative transcriptome analysis reveals key genes associated with meiotic stability and high seed setting rate in tetraploid rice.BMC plant biology · 2025Article
- Lectin Receptor-Like Protein Kinase OsNRFG6 is Required for Embryo Sac Development and Fertilization in Neo-Tetraploid Rice.Rice (New York, N.Y.) · 2024Article
- Cytological Observation and RNA-Seq Analyses RevealPlants (Basel, Switzerland) · 2024Article
- OsmiRNA5488 Regulates the Development of Embryo Sacs and TargetsInternational journal of molecular sciences · 2023Article
- Cytological observation and RNA-seq analysis reveal novel miRNAs high expression associated with the pollen fertility of neo-tetraploid rice.BMC plant biology · 2023Article
- Comparative cytological and transcriptome analyses ofFrontiers in plant science · 2023Article
- An uncharacterized protein NY1 targets EAT1 to regulate anther tapetum development in polyploid rice.BMC plant biology · 2022Article
- Thermo-Sensitive Genic Male Sterile Lines of Neo-Tetraploid Rice Developed through Gene Editing Technology Revealed High Levels of Hybrid Vigor.Plants (Basel, Switzerland) · 2022Article
- Cytological observation and transcriptome analysis reveal dynamic changes ofFrontiers in plant science · 2022Article
- The emerging role of small RNAs in ovule development, a kind of magic.Plant reproduction · 2021Review
- Genome-wide in silico analysis of long intergenic non-coding RNAs from rice peduncles at the heading stage.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- A Genome Doubling Event Reshapes Rice Morphology and Products by Modulating Chromatin Signatures and Gene Expression Profiling.Rice (New York, N.Y.) · 2021Article
- MircroRNA Profiles of Early Rice Inflorescence Revealed a Specific miRNA5506 Regulating Development of Floral Organs and Female Megagametophyte in Rice.International journal of molecular sciences · 2021Article
- A New Way of Rice Breeding: Polyploid Rice Breeding.Plants (Basel, Switzerland) · 2021Review
- Genomics Analyses Reveal Unique Classification, Population Structure and Novel Allele of Neo-Tetraploid Rice.Rice (New York, N.Y.) · 2021Article
- Degradome sequencing-based identification of phasiRNAs biogenesis pathways in Oryza sativa.BMC genomics · 2021Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
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Abstract
backgroundPartial pollen and embryo sac sterilities are the two main reasons for low fertility in autotetraploid rice. Our previous study revealed that small RNAs changes may associate with pollen fertility in autotetraploid rice. However, knowledge on comparative analysis between the development of pollen and embryo sac by small RNAs in autotetraploid rice is still unknown. In the present study, WE-CLSM (whole-mount eosin B-staining confocal laser scanning microscopy) and high-throughput sequencing technology was employed to examine the cytological variations and to analyze small RNAs changes during pollen and embryo sac development in autotetraploid rice compared with its diploid counterpart.
resultsA total of 321 and 368 differentially expressed miRNAs (DEM) were detected during pollen and embryo sac development in autotetraploid rice, respectively. Gene Ontology enrichment analysis on the targets of DEM associated with embryo sac and pollen development revealed 30 prominent functional gene classes, such as cell differentiation and signal transduction during embryo sac development, while only 7 prominent functional gene classes, such as flower development and transcription factor activity, were detected during pollen development in autotetraploid rice. The expression levels of 39 DEM, which revealed interaction with meiosis-related genes, showed opposite expression patterns during pollen and embryo sac development. Of these DEM, osa-miR1436_L + 3_1ss5CT and osa-miR167h-3p were associated with the female meiosis, while osa-miR159a.1 and osa-MIR159a-p5 were related with the male meiosis. 21 nt-phasiRNAs were detected during both pollen and embryo sac development, while 24 nt-phasiRNAs were found only in pollen development, which displayed down-regulation in autotetraploid compared to diploid rice and their spatial-temporal expression patterns were similar to osa-miR2275d. 24 nt TEs-siRNAs were found to be up-regulated in embryo sac but down-regulated in pollen development.
conclusionThe above results not only provide the small RNAs changes during four landmark stages of pollen and embryo sac development in autotetraploid rice but also have identified specifically expressed miRNAs, especially meiosis-related miRNAs, pollen-specific-24 nt-phasiRNAs and TEs-siRNAs in autotetraploid rice. Together, these findings provide a foundation for understanding the effect of polyploidy on small RNAs expression patterns during pollen and embryo sac development that may lead to different abnormalities in autotetraploid rice.
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