ArticleBMC genetics2017
Protanopia (red color-blindness) in medaka: a simple system for producing color-blind fish and testing their spectral sensitivity.
Article in BMC genetics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Visual pigments of basal lineages of bony fishes support independent ecological shifts from a shallow marine to a freshwater niche.Evolution letters · 2026Article
- Temporal dynamics of courtship and spawning in medaka under laboratory conditions revealed by 24 h video monitoring.Scientific reports · 2025Article
- Courtship and spawning behaviour of medaka in a semi-outdoor environment initiating at midnight.Scientific reports · 2025Article
- Medaka (Oryzias latipes) initiate courtship and spawning late at night: Insights from field observations.PloS one · 2025Article
- Behavioral photosensitivity of multi-color-blind medaka: enhanced response under ultraviolet light in the absence of short-wavelength-sensitive opsins.BMC neuroscience · 2023Article
- An Attempt to Identify the Medaka Receptor for Somatolactin Alpha Using a Reverse Genetics Approach.Genes · 2023Article
- Retinal Cone Mosaic in sws1-Mutant Medaka (Oryzias latipes), A Teleost.Investigative ophthalmology & visual science · 2022Article
- Molecular Evolution of Ultraviolet Visual Opsins and Spectral Tuning of Photoreceptors in Anemonefishes (Amphiprioninae).Genome biology and evolution · 2021Article
- Behavioural red-light sensitivity in fish according to the optomotor response.Royal Society open science · 2021Article
- CRISPR/Cas9-mediated generation of biallelic F0 anemonefish (Amphiprion ocellaris) mutants.PloS one · 2021Article
- Seeing the rainbow: mechanisms underlying spectral sensitivity in teleost fishes.The Journal of experimental biology · 2020Review
- Changes in a Cone Opsin Repertoire Affect Color-Dependent Social Behavior in Medaka but Not Behavioral Photosensitivity.Frontiers in genetics · 2020Article
- Evolutionary history of the medaka long-wavelength sensitive genes and effects of artificial regression by gene loss on behavioural photosensitivity.Scientific reports · 2019Article
- Applications of Genome Editing Technology in Animal Disease Modeling and Gene Therapy.Computational and structural biotechnology journal · 2019Review
- A semi-automatic and quantitative method to evaluate behavioral photosensitivity in animals based on the optomotor response (OMR).Biology open · 2018Article
- Review
- Dynamic plasticity in phototransduction regulates seasonal changes in color perception.Nature communications · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundColor perception is important for fish to survive and reproduce in nature. Visual pigments in the retinal photoreceptor cells are responsible for receiving light stimuli, but the function of the pigments in vivo has not been directly investigated in many animals due to the lack of color-blind lines and appropriate color-perception tests.
methodsIn this study, we established a system for producing color-blind fish and testing their spectral sensitivity. First, we disrupted long-wavelength-sensitive (LWS) opsins of medaka (Oryzias latipes) using the CRISPR/Cas9 system to make red-color-blind lines. Single guide RNAs were designed using the consensus sequences between the paralogous LWSa and LWSb genes to simultaneously introduce double-frameshift mutations. Next, we developed a non-invasive and no-prior-learning test for spectral sensitivity by applying an optomotor response (OMR) test under an Okazaki Large Spectrograph (OLS), termed the O-O test. We constructed an electrical-rotary cylinder with black/white stripes, into which a glass aquarium containing one or more fish was placed under various monochromatic light conditions. The medaka were irradiated by the OLS every 10 nm, from wavelengths of 700 nm to 900 nm, and OMR was evaluated under each condition.
resultsWe confirmed that the lws
conclusionsThese results suggest that the lws
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.