ArticlePloS one2017
Characterization of three-dimensional cancer cell migration in mixed collagen-Matrigel scaffolds using microfluidics and image analysis.
Article in PloS one, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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Who cites it
81 citing papers in PubMed.
- Evaluation of Starch-Derived Hydrogel Systems for Artifact-Cleaning Applications.Gels (Basel, Switzerland) · 2026Article
- Vimentin promotes collective cell migration through collagen networks via increased matrix remodeling and spheroid fluidity.Communications biology · 2026Article
- Non-linear Characterization of Commercial and Decellularized Hydrogels: Statistical Framework Enhanced by Bayesian Optimization.Cellular and molecular bioengineering · 2026Article
- Multi-Component 3D Bioprinted Platform with Sacrificial Matrix and Collagen-Based Bioinks for Skeletal Muscle Tissue Engineering.Polymers · 2026Article
- [Study on the establishment, assessment, and drug sensitivity testing for intrahepatic cholangiocarcinoma organoid models based on the suspension culture method].Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology · 2026Article
- Amoeboid-mesenchymal transition and the proteolytic control of cancer invasion plasticity.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Compression-Tension-Asymmetry and Stiffness Nonlinearity of Collagen-Matrigel Composite Hydrogels.Advanced healthcare materials · 2026Article
- Comparative analysis of molecular targeted radiosensitizers in 2D and 3D cancer cell line models.Acta oncologica (Stockholm, Sweden) · 2025Article
- Gelatin Methacrylate Macroporous Cell Scaffold Fabrication via One-Pot Aqueous Two-Phase Separation.Journal of biomedical materials research. Part B, Applied biomaterials · 2025Article
- Modeling Uterine Fibroids Using Bioengineered Hydrogels.ACS biomaterials science & engineering · 2025Article
- Tumor Organoids Grown in Mixed-Composition Hydrogels Recapitulate the Plasticity of Pancreatic Cancers.Gels (Basel, Switzerland) · 2025Article
- Direct visualization of emergent metastatic features within an ex vivo model of the tumor microenvironment.Life science alliance · 2025Article
- Altered Mechanobiology of PDAC Cells with Acquired Chemoresistance to Gemcitabine and Paclitaxel.Cancers · 2024Article
- Targeted Cancer Therapy-on-A-Chip.Advanced healthcare materials · 2024Review
- COMethods and protocols · 2024Article
- Photo-Cross-linked Gelatin Methacryloyl Hydrogels Enable the Growth of Primary Human Endometrial Stromal Cells and Epithelial Gland Organoids.ACS applied materials & interfaces · 2024Article
- Anaplastic thyroid cancer spheroids as preclinical models to test therapeutics.Journal of experimental & clinical cancer research : CR · 2024Article
- Microfluidic model of the alternative vasculature in neuroblastoma.In vitro models · 2024Article
- Comparison of primary and passaged tumor cell cultures and their application in personalized medicine.Exploration of targeted anti-tumor therapy · 2024Review
- Experimental measurement and numerical modeling of deformation behavior of breast cancer cells passing through constricted microfluidic channels.Microsystems & nanoengineering · 2024Article
21 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
14 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microfluidic devices are becoming mainstream tools to recapitulate in vitro the behavior of cells and tissues. In this study, we use microfluidic devices filled with hydrogels of mixed collagen-Matrigel composition to study the migration of lung cancer cells under different cancer invasion microenvironments. We present the design of the microfluidic device, characterize the hydrogels morphologically and mechanically and use quantitative image analysis to measure the migration of H1299 lung adenocarcinoma cancer cells in different experimental conditions. Our results show the plasticity of lung cancer cell migration, which turns from mesenchymal in collagen only matrices, to lobopodial in collagen-Matrigel matrices that approximate the interface between a disrupted basement membrane and the underlying connective tissue. Our quantification of migration speed confirms a biphasic role of Matrigel. At low concentration, Matrigel facilitates migration, most probably by providing a supportive and growth factor retaining environment. At high concentration, Matrigel slows down migration, possibly due excessive attachment. Finally, we show that antibody-based integrin blockade promotes a change in migration phenotype from mesenchymal or lobopodial to amoeboid and analyze the effect of this change in migration dynamics, in regards to the structure of the matrix. In summary, we describe and characterize a robust microfluidic platform and a set of software tools that can be used to study lung cancer cell migration under different microenvironments and experimental conditions. This platform could be used in future studies, thus benefitting from the advantages introduced by microfluidic devices: precise control of the environment, excellent optical properties, parallelization for high throughput studies and efficient use of therapeutic drugs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.