ArticleAnnals of surgery2018
Oral Polyphosphate Suppresses Bacterial Collagenase Production and Prevents Anastomotic Leak Due to Serratia marcescens and Pseudomonas aeruginosa.
Article in Annals of surgery, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
43 citing papers in PubMed, 74 citations in OpenAlex.
- Modulation of the gut microbiota as a novel strategy to prevent anastomotic leak after colorectal surgery: Systematic scoping review.Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland · 2026Article
- Perioperative microbiota at the colorectal anastomosis: local host-microbe interactions in healing and leak.Frontiers in surgery · 2026Review
- Perioperative changes in the microbiome during rectal cancer surgery: exploratory analysis of the National Institute for Health and Care Research (NIHR) IntAct trial.The British journal of surgery · 2025Article
- Fusobacterium nucleatum promotes anastomotic leakage by activating epithelial cells through the NOD1/RIPK2/ERK signalling pathway to drive IL-1β-induced neutrophil chemotaxis and collagen degradation.Clinical and translational medicine · 2025Article
- The Combined Effect of Western Diet Consumption and Diclofenac Administration Alters the Gut Microbiota and Promotes Anastomotic Leakage in the Distal Colon.Biomedicines · 2024Article
- The Effect of Local Papaverine Use in an Experimental High-Risk Colonic Anastomosis Model: Reduced Inflammatory Findings and Less Necrosis.Journal of clinical medicine · 2024Article
- Vitamin B12 ameliorates gut epithelial injury via modulating the HIF-1 pathway and gut microbiota.Cellular and molecular life sciences : CMLS · 2024Article
- The gut barrier as a gatekeeper in colorectal cancer treatment.Oncotarget · 2024Review
- Role of the gut microbiota in tumorigenesis and treatment.Theranostics · 2024Review
- Diet-induced shifts in the gut microbiota influence anastomotic healing in a murine model of colonic surgery.Gut microbes · 2023Article
- Oral antibiotics and mechanical bowel preparation for colorectal surgery: A prospective observational study of surgical site infection and microbial analysis.International journal of colorectal disease · 2023Observational
- Selective Decontamination with Oral Antibiotics in Colorectal Surgery: 90-day Reintervention Rates and Long-term Oncological Follow-up.Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract · 2023Article
- Preparing the Bowel (Microbiome) for Surgery: Surgical Bioresilience.Clinics in colon and rectal surgery · 2023Review
- The Influence of the Microbiome on Anastomotic Leak.Clinics in colon and rectal surgery · 2023Review
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- Antibiotic Bowel Decontamination in Gastrointestinal Surgery-A Single-Center 20 Years' Experience.Frontiers in surgery · 2022Article
- New understanding of gut microbiota and colorectal anastomosis leak: A collaborative review of the current concepts.Frontiers in cellular and infection microbiology · 2022Review
- Mucosa plication reinforced colorectal anastomosis and trans-anal vacuum drainage: a pilot study with preliminary results.Updates in surgery · 2021Article
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Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
objectiveThe objective of this study was to determine the effect of polyphosphate on intestinal bacterial collagenase production and anastomotic leak in mice undergoing colon surgery.
backgroundWe have previously shown that anastomotic leak can be caused by intestinal pathogens that produce collagenase. Because bacteria harbor sensory systems to detect the extracellular concentration of phosphate which controls their virulence, we tested whether local phosphate administration in the form of polyphosphate could attenuate pathogen virulence and prevent leak without affecting bacterial growth.
methodsGroups of mice underwent a colorectal anastomosis which was then exposed to collagenolytic strains of either Serratia marcescens or Pseudomonas aeruginosa via enema. Mice were then randomly assigned to drink water or water supplemented with a 6-mer of polyphosphate (PPi-6). All mice were sacrificed on postoperative day 10 and anastomoses assessed for leakage, the presence of collagenolytic bacteria, and anastomotic PPi-6 concentration.
resultsPPi-6 markedly attenuated collagenase and biofilm production, and also swimming and swarming motility in both S. marcescens and P. aeruginosa while supporting their normal growth. Mice drinking PPi-6 demonstrated increased levels of PPi-6 and decreased colonization of S. marcescens and P. aeruginosa, and collagenase activity at anastomotic tissues. PPi-6 prevented anastomotic abscess formation and leak in mice after anastomotic exposure to S. marcescens and P. aeruginosa.
conclusionsPolyphosphate administration may be an alternative approach to prevent anastomotic leak induced by collagenolytic bacteria with the advantage of preserving the intestinal microbiome and its colonization resistance.
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