ArticleScientific reports2017
Novel flow cytometry approach to identify bronchial epithelial cells from healthy human airways.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Negative regulation of human IL-33 in endothelium during allergic airway inflammation.JCI insight · 2026Article
- Standardized pipeline for establishing, expanding, and differentiating airway and alveolar organoids from human BAL fluid.American journal of physiology. Lung cellular and molecular physiology · 2026Article
- Navigating the Landscape of Cytometry-Based Single-Cell Proteomics: Quantification, Annotation, and Resources.International journal of molecular sciences · 2026Review
- Establishment and characterization of three canine ameloblastoma cell lines with genomic and transcriptomic profiles.PloS one · 2026Article
- Generation of a human vascularized 3D airway model replicating native mucosal heterogeneity.Frontiers in bioengineering and biotechnology · 2026Article
- scANMF: Prior Knowledge and Graph-Regularized NMF for Accurate Cell Type Annotation in scRNA-seq.International journal of molecular sciences · 2025Article
- Ciliated epithelial cell differentiation at air-liquid interface and respiratory syncytial virus infection using animal-free media and substrates.ERJ open research · 2025Article
- Standardized pipeline for establishing, expanding, and differentiating airway and alveolar organoids from human BAL fluid.bioRxiv : the preprint server for biology · 2025Article
- A novelThe European respiratory journal · 2024Article
- A self-training interpretable cell type annotation framework using specific marker gene.Bioinformatics (Oxford, England) · 2024Article
- IL-13 induces loss of CFTR in ionocytes and reduces airway epithelial fluid absorption.The Journal of clinical investigation · 2024Article
- Glycogen synthase kinase 3 inhibition controlsiScience · 2024Article
- Human Airway and Alveolar Organoids from BAL Fluid.American journal of respiratory and critical care medicine · 2024Article
- Fully-automated and ultra-fast cell-type identification using specific marker combinations from single-cell transcriptomic data.Nature communications · 2022Article
- Sputum analysis by flow cytometry; an effective platform to analyze the lung environment.PloS one · 2022Article
- Flow-Cytometric Analysis and Purification of Airway Epithelial-Cell Subsets.American journal of respiratory cell and molecular biology · 2021Article
- From Submerged Cultures to 3D Cell Culture Models: Evolution of Nasal Epithelial Cells in Asthma Research and Virus Infection.Viruses · 2021Review
- House Dust Mite Exposure Causes Increased Susceptibility of Nasal Epithelial Cells to Adenovirus Infection.Viruses · 2020Article
- The Effects of Indoor Pollutants Exposure on Allergy and Lung Inflammation: An Activation State of Neutrophils and Eosinophils in Sputum.International journal of environmental research and public health · 2020Article
- Saliva cell type DNA methylation reference panel for epidemiological studies in children.EpigeneticsArticle
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sampling various compartments within the lower airways to examine human bronchial epithelial cells (HBEC) is essential for understanding numerous lung diseases. Conventional methods to identify HBEC in bronchoalveolar lavage (BAL) and wash (BW) have throughput limitations in terms of efficiency and ensuring adequate cell numbers for quantification. Flow cytometry can provide high-throughput quantification of cell number and function in BAL and BW samples, while requiring low cell numbers. To date, a flow cytometric method to identify HBEC recovered from lower human airway samples is unavailable. In this study we present a flow cytometric method identifying HBEC as CD45 negative, EpCAM/pan-cytokeratin (pan-CK) double-positive population after excluding debris, doublets and dead cells from the analysis. For validation, the HBEC panel was applied to primary HBEC resulting in 98.6% of live cells. In healthy volunteers, HBEC recovered from BAL (2.3% of live cells), BW (32.5%) and bronchial brushing samples (88.9%) correlated significantly (p = 0.0001) with the manual microscopy counts with an overall Pearson correlation of 0.96 across the three sample types. We therefore have developed, validated, and applied a flow cytometric method that will be useful to interrogate the role of the respiratory epithelium in multiple lung diseases.
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