ArticleScientific reports2017
Acellular Urethra Bioscaffold: Decellularization of Whole Urethras for Tissue Engineering Applications.
Article in Scientific reports, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 70 citations in OpenAlex.
- Two phase I/II clinical trials for the treatment of urinary incontinence with autologous mesenchymal stem cells.Stem cells translational medicine · 2020Trial
- Regenerative Medicine Approaches to Stress Urinary Incontinence.Biomimetics (Basel, Switzerland) · 2026Review
- Ex vivo perfusion recellularization of acellular rat hindlimbs for vascular composite allotransplantation.Scientific reports · 2025Article
- Development of UROGRAFT: A Bladder Acellular Matrix-Based Composite for Advanced Cystoplasty, Highlighting the Role of Graft Shape and Composition.ACS biomaterials science & engineering · 2025Article
- Extracellular matrix from decellularized porcine organs as scaffolds for insulin-secreting cells and pancreatic islets.Frontiers in endocrinology · 2025Article
- Decellularization of various tissues and organs through chemical methods.Tissue & cell · 2024Review
- Effects of Two Decellularization Protocols on the Mechanical Behavior and Structural Properties of the Human Urethra.International journal of molecular sciences · 2024Article
- Assessing the effects of bladder decellularization protocols on extracellular matrix (ECM) structure, mechanics, and biology.Journal of pediatric urology · 2024Article
- Response regarding "Assessing the effects of bladder decellularization protocols on extracellular matrix (ECM) structure, mechanics, and biology".Journal of pediatric urology · 2024Article
- Response regarding "Assessing the effects of bladder decellularization protocols on extracellular matrix (ECM) structure, mechanics, and biology".Journal of pediatric urology · 2024Article
- Follow-up and histocompatibility observation of urethral reconstruction with different materials.American journal of clinical and experimental urology · 2024Article
- Injectable Decellularized Extracellular Matrix-Based Bio-Ink with Excellent Biocompatibility for Scarless Urethra Repair.Gels (Basel, Switzerland) · 2023Article
- Decellularization techniques of human foreskin for tissue engineering application.Physiological research · 2023Article
- Decellularization of the human urethra for tissue engineering applications.Experimental biology and medicine (Maywood, N.J.) · 2023Article
- Impact of various detergent-based immersion and perfusion decellularization strategies on the novel caprine pancreas derived extracellular matrix scaffold.Frontiers in bioengineering and biotechnology · 2023Article
- Development potential of extracellular matrix hydrogels as hemostatic materials.Frontiers in bioengineering and biotechnology · 2023Review
- Preparation and Use of Decellularized Extracellular Matrix for Tissue Engineering.Journal of functional biomaterials · 2022Review
- Therapies Based on Adipose-Derived Stem Cells for Lower Urinary Tract Dysfunction: A Narrative Review.Pharmaceutics · 2022Review
- Spleen extracellular matrix provides a supportive microenvironment for β-cell function.Iranian journal of basic medical sciences · 2022Article
- Tailor-made natural and synthetic grafts for precise urethral reconstruction.Journal of nanobiotechnology · 2022Review
Corrections and comments
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Authors and funding
12 authors at 6 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Patients with stress urinary incontinence mainly suffer from malfunction of the urethra closure mechanism. We established the decellularization of porcine urethras to produce acellular urethra bioscaffolds for future tissue engineering applications, using bioscaffolds or bioscaffold-derived soluble products. Cellular removal was evaluated by H&E, DAPI and DNA quantification. The presence of specific ECM proteins was assessed through immunofluorescence staining and colorimetric assay kits. Human skeletal muscle myoblasts, muscle progenitor cells and adipose-derived stromal vascular fractions were used to evaluate the recellularization of the acellular urethra bioscaffolds. The mechanochemical decellularization system removed ~93% of tissue's DNA, generally preserving ECM's components and microarchitecture. Recellularization was achieved, though methodological advances are required regarding cell seeding strategies and functional assessment. Through microdissection and partial digestion, different urethra ECM-derived coating substrates were formulated (i.e. containing smooth or skeletal muscle ECM) and used to culture MPCs in vitro. The skeletal muscle ECM substrates enhanced fiber formation leading to the expression of the main skeletal muscle-related proteins and genes, as confirmed by immunofluorescence and RT-qPCR. The described methodology produced a urethra bioscaffold that retained vital ECM proteins and was liable to cell repopulation, a crucial first step towards the generation of urethra bioscaffold-based Tissue Engineering products.
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