ArticleThe Journal of reproduction and development2017
Effects of exosome-like vesicles on cumulus expansion in pigs in vitro.
Article in The Journal of reproduction and development, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 47 citations in OpenAlex.
- Sex differences in urinary extracellular vesicles originating from the genitourinary system in health and disease.American journal of physiology. Renal physiology · 2026Review
- Extracellular vesicles in monitoring and modulation of oocyte competence: focus on exosomes.Journal of ovarian research · 2025Review
- Impact of Neutral Sphingomyelinase Inhibition on Small Extracellular Vesicle Production by Mural Granulosa Cells and In Vitro Folliculogenesis in Mice.Molecular reproduction and development · 2025Article
- Small and Large Extracellular Vesicles From Human Preovulatory Follicular Fluid Display Distinct ncRNA Cargo Profiles and Differential Effects on KGN Granulosa Cells.Journal of extracellular vesicles · 2025Article
- Extracellular vesicles: key mediators inFrontiers in veterinary science · 2025Review
- Extracellular vesicles and their content in the context of polycystic ovarian syndrome and endometriosis: a review.Journal of ovarian research · 2024Review
- Porcine Granulosa-Cell-Derived Exosomes Enhance Oocyte Development: An In Vitro Study.Antioxidants (Basel, Switzerland) · 2024Article
- Follicular Fluid-Derived Extracellular Vesicles Influence on In Vitro Maturation of Equine Oocyte: Impact on Cumulus Cell Viability, Expansion and Transcriptome.International journal of molecular sciences · 2024Article
- Effect of Exosomes Derived from Bone Marrow Mesenchymal Stem Cells on Ovarian Granulosa Cells of Immature NMRI Mice.Cell journal · 2024Article
- The function of exosomes in ovarian granulosa cells.Cell and tissue research · 2023Review
- Seminal extracellular vesicles subsets modulate gene expression in cumulus cells of porcine in vitro matured oocytes.Scientific reports · 2022Article
- Extracellular Vesicles in Veterinary Medicine.Animals : an open access journal from MDPI · 2022Review
- Biomaterials and advanced technologies for the evaluation and treatment of ovarian aging.Journal of nanobiotechnology · 2022Review
- Small-extracellular vesicles and their microRNA cargo from porcine follicular fluids: the potential association with oocyte quality.Journal of animal science and biotechnology · 2022Article
- Messenger roles of extracellular vesicles during fertilization of gametes, development and implantation: Recent advances.Frontiers in cell and developmental biology · 2022Review
- Comparison of MicroRNA Profiles in Extracellular Vesicles from Small and Large Goat Follicular Fluid.Animals : an open access journal from MDPI · 2021Article
- In Vitro Production of Embryos from Prepubertal Holstein Cattle and Mediterranean Water Buffalo: Problems, Progress and Potential.Animals : an open access journal from MDPI · 2021Review
- Exosomes as Naturally Occurring Vehicles for Delivery of Biopharmaceuticals: Insights from Drug Delivery to Clinical Perspectives.Nanomaterials (Basel, Switzerland) · 2021Review
- Extracellular vesicles and female reproduction.Journal of assisted reproduction and genetics · 2021Review
- Activation-induced cytidine deaminase is a possible regulator of cross-talk between oocytes and granulosa cells through GDF-9 and SCF feedback system.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell-secreted vesicles, such as exosomes, have recently been recognized as mediators of cell communication. A recent study in cattle showed the involvement of exosome-like vesicles in the control of cumulus expansion, a prerequisite process for normal ovulation; however, whether this is the case in other mammalian species is not known. Therefore, this study aimed to examine the presence of exosome-like vesicles in ovarian follicles and their effects on cumulus expansion in vitro in pigs. The presence of exosome-like vesicles in porcine follicular fluid (pFF) was confirmed by transmission electron microscopic observation, the detection of marker proteins, and RNA profiles specific to exosomes. Fluorescently labeled exosome-like vesicles isolated from pFF were incorporated into both cumulus and mural granulosa cells in vitro. Exosome-like vesicles were not capable of inducing cumulus expansion to a degree comparable to that induced by follicle-stimulating hormone (FSH). Moreover, exosome-like vesicles had no significant effects on the expression levels of transcripts required for the normal expansion process (HAS2, TNFAIP6, and PTGS2). Interestingly, FSH-induced expression of HAS2 and TNFAIP6 mRNA, but not of PTGS2 mRNA, was significantly increased by the presence of exosome-like vesicles; however, the degree of FSH-induced expansion was not affected. In addition, porcine exosome-like vesicles had no significant effects on the expansion of mouse cumulus-oocyte complexes. Collectively, the present results suggest that exosome-like vesicles are present in pFF, but they are not efficient in inducing cumulus expansion in pigs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.