ArticleCell chemical biology2017
Discovering Targets of Non-enzymatic Acylation by Thioester Reactivity Profiling.
Article in Cell chemical biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 99 citations in OpenAlex.
- Lactylation at the metabolic-epigenetic interface in cardiovascular diseases: context-dependent mechanisms and translational roadmap.Journal of advanced research · 2026Review
- Processes and therapeutic perspectives of acylation modifications of lysine and cysteine in tumors.Cell communication and signaling : CCS · 2026Review
- Successful axonal regeneration is associated with intraneuronal metabolic reprogramming.iScience · 2025Article
- Thio-NHS esters are non-innocent protein acylating reagents.Nature communications · 2025Article
- Mechanisms of metabolism-coupled protein modifications.Nature chemical biology · 2025Review
- Malonate promotes CD8Cellular & molecular immunology · 2025Article
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- Propionylation of Fis K32 inFuture microbiology · 2025Article
- Chemical catalyst manipulating cancer epigenome and transcription.Nature communications · 2025Article
- Acetyl-CoA synthetase activity is enzymatically regulated by lysine acetylation using acetyl-CoA or acetyl-phosphate as donor molecule.Nature communications · 2024Article
- Histone acylation at a glance.Journal of cell science · 2024Review
- A global view of the human post-translational modification landscape.The Biochemical journal · 2023Review
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- New insights into the pathophysiology of methylmalonic acidemia.Journal of inherited metabolic disease · 2023Review
- Histone malonylation is regulated by SIRT5 and KAT2A.iScience · 2023Article
- Protein-metabolite interactomics of carbohydrate metabolism reveal regulation of lactate dehydrogenase.Science (New York, N.Y.) · 2023Article
- Lysine Malonylation and Its Links to Metabolism and Diseases.Aging and disease · 2023Review
- The 2022 Nobel Prize in Chemistry for the development of click chemistry and bioorthogonal chemistry.Analytical and bioanalytical chemistry · 2023Article
- Activity of Fatty Acid Biosynthesis Initiating Ketosynthase FabH with Acetyl/Malonyl-oxa/aza(dethia)CoAs.ACS chemical biology · 2023Article
- Biochemical evidence that the whole compartment activity behavior of GAPDH differs between the cytoplasm and nucleus.PloS one · 2023Article
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Authors and funding
20 authors at 8 institutions in 1 country.
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Abstract
Non-enzymatic protein modification driven by thioester reactivity is thought to play a major role in the establishment of cellular lysine acylation. However, the specific protein targets of this process are largely unknown. Here we report an experimental strategy to investigate non-enzymatic acylation in cells. Specifically, we develop a chemoproteomic method that separates thioester reactivity from enzymatic utilization, allowing selective enrichment of non-enzymatic acylation targets. Applying this method to cancer cell lines identifies numerous candidate targets of non-enzymatic acylation, including several enzymes in lower glycolysis. Functional studies highlight malonyl-CoA as a reactive thioester metabolite that can modify and inhibit glycolytic enzyme activity. Finally, we show that synthetic thioesters can be used as novel reagents to probe non-enzymatic acylation in living cells. Our studies provide new insights into the targets and drivers of non-enzymatic acylation, and demonstrate the utility of reactivity-based methods to experimentally investigate this phenomenon in biology and disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.