ArticlePlanta2017
Next-generation sequencing of representational difference analysis products for identification of genes involved in diosgenin biosynthesis in fenugreek (Trigonella foenum-graecum).
Article in Planta, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 57 citations in OpenAlex.
- Comparative Transcriptomic Analysis Provides Insights into PSK-α-Mediated Regulation of Diosgenin Biosynthesis inMolecules (Basel, Switzerland) · 2026Article
- Identification of candidate genes governing key metabolic pathways in fenugreek (Trigonella spp.) through integrated transcriptomic and metabolomic analysis.Functional & integrative genomics · 2026Article
- Article
- Transcriptomic data reveals the dynamics of terpenoids biosynthetic pathway of fenugreek.BMC genomics · 2024Article
- Differential responses of two fenugreek (Trigonella foenum-graecum L.) landraces pretreated with melatonin to prolonged drought stress and subsequent recovery.BMC plant biology · 2024Article
- Identification of candidate genes responsible for chasmogamy in wheat.BMC genomics · 2023Article
- Advances in the Biosynthesis and Molecular Evolution of Steroidal Saponins in Plants.International journal of molecular sciences · 2023Review
- Identification of key genes involved in secondary metabolite biosynthesis in Digitalis purpurea.PloS one · 2023Article
- Comparative transcriptomics and metabolomics reveal specialized metabolite drought stress responses in switchgrass (Panicum virgatum).The New phytologist · 2022Article
- Exploring the medicinally important secondary metabolites landscape through the lens of transcriptome data in fenugreek (Trigonella foenum graecum L.).Scientific reports · 2022Article
- Identification and a phased pH control strategy of diosgenin bio-synthesized by an endogenous Bacillus licheniformis Syt1 derived from Dioscorea zingiberensis C. H. Wright.Applied microbiology and biotechnology · 2021Article
- Advances in steroidal saponins biosynthesis.Planta · 2021Review
- Omics Technologies to Enhance Plant Based Functional Foods: An Overview.Frontiers in genetics · 2021Review
- Review
- High-Density SNP-Based Association Mapping of Seed Traits in Fenugreek Reveals Homology with Clover.Genes · 2020Article
- Representational Difference Analysis of Transcripts Involved in Jervine Biosynthesis.Life (Basel, Switzerland) · 2020Article
- Comparative Transcriptome Analysis Identifies Genes Involved in Diosgenin Biosynthesis inMolecules (Basel, Switzerland) · 2019Article
- Review
- Pro-Apoptotic and Anti-Cancer Properties of Diosgenin: A Comprehensive and Critical Review.Nutrients · 2018Review
- Identification, characterization and expression analysis of genes involved in steroidal saponin biosynthesis in Dracaena cambodiana.Journal of plant research · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MAIN
conclusionRepresentational difference analysis of cDNA was performed and differential products were sequenced and annotated. Candidate genes involved in biosynthesis of diosgenin in fenugreek were identified. Detailed mechanism of diosgenin synthesis was proposed. Fenugreek (Trigonella foenum-graecum L.) is a valuable medicinal and crop plant. It belongs to Fabaceae family and has a unique potential to synthesize valuable steroidal saponins, e.g., diosgenin. Elicitation (methyl jasmonate) and precursor feeding (cholesterol and squalene) were used to enhance the content of sterols and steroidal sapogenins in in vitro grown plants for representational difference analysis of cDNA (cDNA-RDA). To identify candidate genes involved in diosgenin biosynthesis, differential, factor-specific libraries were subject to the next-generation sequencing. Approximately 9.9 million reads were obtained, trimmed, and assembled into 31,491 unigenes with an average length of 291 bp. Then, functional annotation and gene ontogeny enrichment analysis was performed by aligning all-unigenes with public databases. Within the transcripts related to sterol and steroidal saponin biosynthesis, we discovered novel candidate genes of diosgenin biosynthesis and validated their expression using quantitative RT-PCR analysis. Based on these findings, we supported the idea that diosgenin is biosynthesized from cycloartenol via cholesterol. This is the first report on the next-generation sequencing of cDNA-RDA products. Analysis of the transcriptomes enriched in low copy sequences contributed substantially to our understanding of the biochemical pathways of steroid synthesis in fenugreek.
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