ArticleInternational journal for parasitology2017
The genome of the protozoan parasite Cystoisospora suis and a reverse vaccinology approach to identify vaccine candidates.
Article in International journal for parasitology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 41 citations in OpenAlex.
- The Major Facilitator Superfamily Transporter HAP12 Is Critical inInternational journal of molecular sciences · 2025Article
- Deciphering the code of resistance: a genomic and transcriptomic exploration of the Cystoisospora suis Holland-I strain.Scientific reports · 2025Article
- A virus associated with the zoonotic pathogenbioRxiv : the preprint server for biology · 2024Article
- A virus associated with the zoonotic pathogenVirus evolution · 2024Article
- Development of a new quantification method of Sarcocystis cruzi through detection of the acetyl-CoA synthetase gene.The Journal of veterinary medical science · 2023Article
- Mobilome of Apicomplexa Parasites.Genes · 2022Article
- The transcriptome from asexual to sexual in vitro development of Cystoisospora suis (Apicomplexa: Coccidia).Scientific reports · 2022Article
- A reverse vaccinology approach identifies putative vaccination targets in the zoonotic nematodeFrontiers in veterinary science · 2022Article
- Complete genomes of the eukaryotic poultry parasite Histomonas meleagridis: linking sequence analysis with virulence / attenuation.BMC genomics · 2021Article
- Sexual Development in Non-Human Parasitic Apicomplexa: Just Biology or Targets for Control?Animals : an open access journal from MDPI · 2021Review
- T Cell Peptides Derived from Invasive Stages ofJournal of clinical medicine · 2021Article
- Next-Generation Technologies and Systems Biology for the Design of Novel Vaccines Against Apicomplexan Parasites.Frontiers in veterinary science · 2021Review
- ExploringGenes · 2020Review
- Microsatellite Analysis of Geographically Close Isolates ofFrontiers in veterinary science · 2019Article
- Molecular characterisation and the protective immunity evaluation of Eimeria maxima surface antigen gene.Parasites & vectors · 2018Article
- Genomic and Proteomic Evidence for the Presence of a Peroxisome in the Apicomplexan Parasite Toxoplasma gondii and Other Coccidia.Genome biology and evolution · 2017Article
- Cloning, expression and molecular characterization of a Cystoisospora suis specific uncharacterized merozoite protein.Parasites & vectors · 2017Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Vaccine development targeting protozoan parasites remains challenging, partly due to the complex interactions between these eukaryotes and the host immune system. Reverse vaccinology is a promising approach for direct screening of genome sequence assemblies for new vaccine candidate proteins. Here, we applied this paradigm to Cystoisospora suis, an apicomplexan parasite that causes enteritis and diarrhea in suckling piglets and economic losses in pig production worldwide. Using Next Generation Sequencing we produced an ∼84Mb sequence assembly for the C. suis genome, making it the first available reference for the genus Cystoisospora. Then, we derived a manually curated annotation of more than 11,000 protein-coding genes and applied the tool Vacceed to identify 1,168 vaccine candidates by screening the predicted C. suis proteome. To refine the set of candidates, we looked at proteins that are highly expressed in merozoites and specific to apicomplexans. The stringent set of candidates included 220 proteins, among which were 152 proteins with unknown function, 17 surface antigens of the SAG and SRS gene families, 12 proteins of the apicomplexan-specific secretory organelles including AMA1, MIC6, MIC13, ROP6, ROP12, ROP27, ROP32 and three proteins related to cell adhesion. Finally, we demonstrated in vitro the immunogenic potential of a C. suis-specific 42kDa transmembrane protein, which might constitute an attractive candidate for further testing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.